TY  - BOOK
AU  - Rehab Ali Mohammed Mohammed Elhelw
AU  - Ahmed Abdelghani Elsanousi , 
AU  - Mahmoud Essam Hatem Ahmed , 
AU  - Mona Ibrahim Elnbaawy , 
TI  - Production of monoclonal antibodies-based ELISA kits for pathogenic Leptospira spp. / 
PY  - 2014///
CY  - Cairo : 
PB  - Rehab Ali Mohammed Mohammed Elhelw , 
KW  - MAbs
KW  - Pathogenic Leptospira spp.
KW  - Serovar Canicola
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology; Issued also as CD
N2  - A panel of monoclonal antibodies (MAbs) against each of Leptospira interrogans serovar Canicola and pathogenic Leptospira spp. were produced. L. interrogans serovar Canicola was cultivated, concentrated and prepared for fusion. BALB/c mice of 6 weeks old were inoculated with different antigenic preparations of L. interrogans serovar Canicola with complete freund{u2019}s adjuvant. Booster doses with and without incomplete freund{u2019}s adjuvant were applied. To obtain hybridomas, mice spleen cells were fused with myeloma cells in the presence of polyethylene glycol. Hybridoma cells that secret specific antibodies were selected on the basis of ELISA. These positive hybridomas were cloned by limiting dilution method to reveal subclones of one cell origin. 5 specific clones were obtained
UR  - http://172.23.153.220/th.pdf
ER  -