Mariam Hassan Abdelmoneam Haikal  <br/><br/>
A study on the production of relevant  Biosurfactant (s) by biodegrading bacterial isolates /  
 دراسة عن إنتاج مركبات تقليل التوتر السطحى  البيولوجية من عزلات بكتيرية ُمكِّسرة  
Mariam Hassan Abdelmoneam Haikal ; Supervised Aisha Abdelaziz Salama , Aymen Samir Yassin , Tamer Mohamed Essam 
 - Cairo :   Mariam Hassan Abdelmoneam Haikal ,   2014 
 - 213 P. :   charts ;   25cm 
<br/><br/>Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of  Microbiology and Immunology  
<br/><br/> A total of nineteen bacterial isolates were screened for their  biodegradation, metabolic versatility and biosurfactants production  using various organic pollutants.  The biosurfactants production ability was mainly assessed by  spread test (OST) and/or emulsification assay (EA). Although  initial biosurfactants screening was conducted using paraffin oil, the  application of vegetable oils, particularly coconut oil, was always  accompanied with the highest yield of biosurfactants production.   Biochemical and molecular identification of the nineteen  isolates revealed that they belong to four genera; Burkholderia (9  isolates), Klebsiella (6 isolates), Pseudomonas (3 isolates) and  Citrobacter (1 isolate).  Interestingly, six isolates (M2H2 1, M2H2 3, M2H2 7, M2H2  M2H2 14 and M2H2 15), showed the highest biosurfactants  production and therefore were further assessed using mixed carbon  source (coconut oil in combination with one organic pollutant  (phenol or cyclohexanol)). The addition of the coconut oil was  essential for increased production of biosurfactant, while the use of  organic pollutant as a sole carbon source was always accompanied  with lower productivity 
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<br/><br/><label>Subjects--Index Terms: </label>Biodegrading bacterial isolates  Relevant  Biosurfactant (s) The biosurfactants