Etiology of leptospirosis in bovine with special reference to preparation of an autogenous vaccine /
مسبب مرض اللبتوسبيروسيس في الابقار مع التركيز علي تحضير لقاح ذاتي للمرض
Eman Shafeek Ibrahim Salam ; Supervised Mahmoud Essam Hatem Ahmed , Ahmed Samir M. Shehata , Nagwa Sayd Ata
- Cairo : Eman Shafeek Ibrahim Salam , 2014
- 105 P. : charts ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
The present study aimed to (i) isolation, serodiagnosis and PCR examination for 200 rat samples, 625 cow samples, 95 human blood samples and 30 water samples. Isolation study showed 9 isolates in rat and 7 isolates for both cow and water source. All isolates serotyped as L. Icterohaemorrhagiae and L. Pomona. The most predominant serovars were against L. Pomona, L. Icterohaemorrhagiae, L. Pyrogenes ,L. Hardjo , L. celledoni, and L. Grippotyphosa . Results of PCR examination revealed that 28 (14%) were positive with lig₁/lig₂ and 58 samples (29%) with G1/G2 in rat samples, 7 (1.12%) with lig1/lig2 and 30 samples (4.8%) with G1/G2 in cow samples and 10 (33%) with lig₁/lig₂ and 30 samples (100%) with G1/G2 in water samples.(ii) second study on vaccine preparation and evaluation; an autogenous whole cell killed bivalent leptospira vaccine formulated with the two recovered isolates (L. Icterohaemorrhagiae and L. Pomona) was evaluated in 20 Guinea pigs vaccinated with 0.25 ml S/C in 1st and 2nd dose after 3 weeks which gave protection and prevent urinary shedding and 6 other Guinea pigs were used as unvaccinated control animal