TY  - BOOK
AU  - Eman Shafeek Ibrahim Salam
AU  - Ahmed Samir M. Shehata , 
AU  - Mahmoud Essam Hatem Ahmed , 
AU  - Nagwa Sayd Ata , 
TI  - Etiology of leptospirosis in bovine with special reference to preparation of an autogenous vaccine / 
PY  - 2014///
CY  - Cairo : 
PB  - Eman Shafeek Ibrahim Salam , 
KW  - Leptospira
KW  - Leptospirosis
KW  - Vaccine
N1  - Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology; Issued also as CD
N2  - The present study aimed to (i) isolation, serodiagnosis and PCR examination for 200 rat samples, 625 cow samples, 95 human blood samples and 30 water samples. Isolation study showed 9 isolates in rat and 7 isolates for both cow and water source. All isolates serotyped as L. Icterohaemorrhagiae and L. Pomona. The most predominant serovars were against L. Pomona,  L. Icterohaemorrhagiae, L. Pyrogenes ,L. Hardjo , L. celledoni, and L. Grippotyphosa . Results of PCR examination revealed that 28 (14%) were positive with lig₁/lig₂ and 58 samples (29%) with G1/G2 in rat samples, 7 (1.12%) with lig1/lig2 and 30 samples (4.8%) with G1/G2 in cow samples and 10 (33%) with lig₁/lig₂ and 30 samples (100%) with G1/G2 in water samples.(ii) second study on vaccine preparation and evaluation; an autogenous whole cell killed bivalent leptospira vaccine formulated with the two recovered isolates (L. Icterohaemorrhagiae and L. Pomona) was evaluated in 20 Guinea pigs vaccinated with 0.25 ml S/C in 1st and 2nd dose after 3 weeks which gave protection and prevent urinary shedding and 6 other Guinea pigs were used as unvaccinated control animal
UR  - http://172.23.153.220/th.pdf
ER  -