TY - BOOK AU - Eman Mohamed Samy Ahmed Elnaggar AU - Fakreya Abdelhafez Albordeny , AU - Mohamed Ali Mohamed Elhady , AU - Salah Eldien Abdelkarim Selim , TI - Study of the immunopotentiation effect of Esat-6 of mycobacteria on DNA vaccine encoding the H5 gene of avian influenza virus / PY - 2014/// CY - Cairo : PB - Eman Mohamed Samy Ahmed Elnaggar , KW - DNA vaccine encoding KW - H5 gene KW - Immunopotentiation N1 - Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology; Issued also as CD N2 - In the current study , the DNA vaccine coding for H5 gene of HPA1 H%N1 subtype was produced and co-administrated with DNA vaccine coding for esat6 gene of Mycobacteria (pcDNA3.1/esat6) as an immunomodulatore. first, samples taken from different governorates during 2010 and 2011 were subjected to extensive virus isolation and identification one of 2011 were subjected to extensive virus isolation and identification one of the isolated feed isolate was subjected for further investigation where the full length H5 gene was amplifed and sequenced and cloned in pENTER/D/SD/Topo entry clone and homologues recombination with pDEST40 mammalian expression destination vector was done to produce the DNA vaccine pDEST40/H5 UR - http://172.23.153.220/th.pdf ER -