Immunological studies on camels (Camelus dromedarius ) in Egypt /
دراسات مناعية على الجمال وحيدة السنام فى مصر
Ehab Ali Mohamed Mohamed Fouad ; Supervised Mahmoud Essam Hatem Ahmed , Jakeen Kamal Abdelhalem Eljakee , Nagwa Sayed Sayed Mohammed Ata
- Cairo : Ehab Ali Mohamed Mohamed Fouad , 2015
- 114 P. : charts ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
As the labeled anti-camel Igs with enzymes for ELISA are unavailable in commercial level, the present investigation was directed for developing labeled anti-camel IgG with horseradish peroxidase. For purification of camel IgG whole molecule, camel sera were preliminary precipitated with 50% saturated ammonium sulphate and dialyzed against diluted PBS then concentrated. This preparation was followed by Protein A Sepharose affinity column chromatograhy for purification of camel IgG. The purity of the eluted camel IgG was tested by SDS-PAGE. Four bands of molecular weight 63, 52, 40 and 33 kDa, represent camel IgG, were detected. Anti-camel IgG was prepared by immunization of goats and rabbits separately, with purified camel IgG. Then anti-camel IgG was purified by loading on Protein A Sepharose affinity column chromatography after precipitation and concentration. Whole molecule anti-camel IgG was conjugated with horseradish peroxidase