TY  - BOOK
AU  - Asmaa Ahmed Abdelghaffar 
AU  - Abeer A. Elhalim Boseila , 
AU  - Amal Emad Eldin , 
AU  - Magdy Ali Amin  , 
TI  - Study of In vitro inactivation of rabies virus by hydrogen peroxide / 
PY  - 2015///
CY  - Cairo : 
PB  - Asmaa Ahmed Abdelghaffar , 
KW  - Hydrogen Peroxide
KW  - Inactivation
KW  - Rabies
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology; Issued also as CD
N2  - Development of safe and protective vaccines against infectious pathogens  remains a challenge. Inactivation of rabies virus is a critical step in the  production of vaccines and other research reagents. Beta-propiolactone  (ÝPL); the currently used inactivating agent for rabies virus is expensive  and proved to be carcinogenic in animals. This study aimed to investigate  the ability of hydrogen peroxide (H₂O₂) to irreversibly inactivate rabies  virus without affecting its antigenicity and immunogenicity in pursuit of  finding safe, effective and inexpensive alternative inactivating agents. H₂O₂  3% rapidly inactivated a Vero cell adapted fixed rabies virus strain  designated as FRV/K within two hours of exposure without affecting  antigenicity or immunogenicity. No residual infectious virus was detected  and the H₂O₂- inactivated vaccine proved to be safe and effective when  compared with the same virus harvest inactivated with the classical  inactivating agent (ÝPL). Mice immunized with H₂O₂-inactivated rabies  virus produced sufficient level of antibodies and were protected when  challenged with lethal CVS virus. These findings reinforce the idea that  H₂O₂ can replace ÝPL as inactivating agent for rabies virus to reduce time  and cost of inactivation process
UR  - http://172.23.153.220/th.pdf
ER  -