01678cam a2200253 a 4500003000900000005001700009008004100026040002800067041000800095100002500103245018800128246015000316260004300466300003500509502009000544520063700634530002201271653001301293653000801306653000901314700003601323700003201359856003301391EG-GiCUC20250223031514.0160525s2015    ua h   f m    000 0 eng d  aEG-GiCUCbengcEG-GiCUC0 aeng0 aMarian Elias Makkar 10aGenetic studies and molecular identification of lymnaea snails (Family Lymnaeidae) in certain governorates in Egypt / cMarian Elias Makkar ; Supervised Sanaa Osman , Samia Elbardicy 15aدراسات جينية و تعريف جزيئي لقواقع ليمنيا (عائلة  ليمنيدي) ببعض المحافظات بمصر   aCairo : bMarian Elias Makkar , c2015  a123 P. : bfacsimiles ; c25cm  aThesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology   aIn the past the anatomical studies were used to distinguish  between different Lymnaea species. In this study RFLP technique was used on a partial sequence of the 18S rDNA  to differentiate between different species of Lymnaea snails  which are the intermediate host of Fasciola. The 18S gene  is part of the ribosomal functional core and recently it is becoming one of the most frequently used genes in  phylogenetic studies and for identification and classification  of living organisms, owing to their repetitive arrangement within the genome as they provide excessive amounts of  template DNA for PCR, even in smallest organisms  aIssued also as CD 4a18S rDNA 4aPCR 4aRFLP0 aSamia Elbardicy  , eSupervisor0 aSanaa Osman  , eSupervisor  uhttp://172.23.153.220/th.pdf