02330cam a2200265 a 4500003000900000005001700009008004100026040002800067041000800095100003400103245022300137246019500360260005300555300003200608502012900640520105700769530002201826653000901848653002101857653002301878700004501901700004701946700003801993856003302031EG-GiCUC20250223032118.0181119s2018    ua d   f m    000 0 eng d  aEG-GiCUCbengcEG-GiCUC0 aeng0 aMahmoud Esmail Mohamed Alalfy10aLaser based detection of maternal plasma cell free fetal DNA for early fetal sex determination / cMahmoud Esmail Mohamed Alalfy ; Supervised Mohamed Amr Hussein Elnoury , Osama Mahmoud Azmy , Jehan Mohamed Alsharnoubi15aالتحديد المبكرلجنس الجنين بإستخدام تقنيه الليزر للحامض النووى الجنينى الحر الموجود فى بلازما دم الأم  aCairo : bMahmoud Esmail Mohamed Alalfy , c2018  a173 P. : bcharts ; c25cm   aThesis (Ph.D.) - Cairo University - National Institute of Laser - Department of Laser Application in Medical and Biological   aEnhancement of DNA detection assays increases the use of  non-invasive prenatal diagnosis that consequently enhances the supervision plan of pregnant woman  with genetic disorder that could have an expected babies with this disorder. Aim of work: current work was planned to examine plasma of  pregnant women starting from 6 th week of the pregnancy for Cell-free fetal DNA,  to determine embryonic sex type with consequent prospect of  detection of fetal anomalies such as sex-linked genetic abnormalities . Method: samples collected from 6 th  week  till 11 th  week  of pregnancy according to pregnancy age   and tested using Real time PCR for determination of fetal sex Results: testing of samples resulted   in detection of   fetal  sex starting from  6th week and the later  the gestational age ,the better the  result  for detection of fetal  sex . all results  were confirmed by Ultrasound  scan  and  neonatal outcome. Testing results revealed PCR detection for 58 males and 92 females with confusion in one  fetus  due to non identical twins   aIssued also as CD 4aDNA  4aMaternal plasma  4aSex determination 0 aJehan Mohamed Alsharnoubi , eSupervised0 aMohamed Amr Hussein Elnoury , eSupervised0 aOsama Mahmoud Azmy , eSupervised  uhttp://172.23.153.220/th.pdf