02533cam a2200349 a 4500 EG-GiCUC 20250223032118.0 181119s2018 ua d f m 000 0 eng d EG-GiCUC eng EG-GiCUC eng Deposite Ph.D Cai01.24.03.Ph.D.2018.Ma.L Mahmoud Esmail Mohamed Alalfy Laser based detection of maternal plasma cell free fetal DNA for early fetal sex determination / Mahmoud Esmail Mohamed Alalfy ; Supervised Mohamed Amr Hussein Elnoury , Osama Mahmoud Azmy , Jehan Mohamed Alsharnoubi التحديد المبكرلجنس الجنين بإستخدام تقنيه الليزر للحامض النووى الجنينى الحر الموجود فى بلازما دم الأم Cairo : Mahmoud Esmail Mohamed Alalfy , 2018 173 P. : charts ; 25cm Thesis (Ph.D.) - Cairo University - National Institute of Laser - Department of Laser Application in Medical and Biological Enhancement of DNA detection assays increases the use of non-invasive prenatal diagnosis that consequently enhances the supervision plan of pregnant woman with genetic disorder that could have an expected babies with this disorder. Aim of work: current work was planned to examine plasma of pregnant women starting from 6 th week of the pregnancy for Cell-free fetal DNA, to determine embryonic sex type with consequent prospect of detection of fetal anomalies such as sex-linked genetic abnormalities . Method: samples collected from 6 th week till 11 th week of pregnancy according to pregnancy age and tested using Real time PCR for determination of fetal sex Results: testing of samples resulted in detection of fetal sex starting from 6th week and the later the gestational age ,the better the result for detection of fetal sex . all results were confirmed by Ultrasound scan and neonatal outcome. Testing results revealed PCR detection for 58 males and 92 females with confusion in one fetus due to non identical twins Issued also as CD DNA Maternal plasma Sex determination Jehan Mohamed Alsharnoubi , Supervised Mohamed Amr Hussein Elnoury , Supervised Osama Mahmoud Azmy , Supervised http://172.23.153.220/th.pdf Nazla Revisor Shimaa Cataloger ddc TH 68619 68619 ddc 0 CUCL cl210 2024-02-11 Cai01.24.03.Ph.D.2018.Ma.L 01010110076375000 2023-09-22 00:00:00 TH ddc 0 CUCL cl2u8 2024-02-11 Cai01.24.03.Ph.D.2018.Ma.L 01020110076375000 2023-09-22 00:00:00 76375.CD CD