Mohammed Aref Marwan Kyyaly

Cloning , overexpression , and characterization of human lung mast cell carboxypeptidase a in the yeast saccharomyces cerevisiae / استنساخ والتعبير عن ودراسة انزيم الكربوكسى ببتيديز من خلايا الرئة البشرية الجذعية فى خميرة الخبز Mohammed Aref Marwan Kyyaly ; Supervised Sayed Kamel Goda - Cairo : Mohammed Aref Marwan Kyyaly , 2006 - 89P : diagrs ; 30cm

Thesis (M.Sc.) - Cairo University - Faculty Of Science - Department Of Biochemistry

Human skin and lung mast cells contain a carboxypeptidase in their secretory granulesThe biological function of carboxypeptidase A , CPA is uncertain but previous studies suggested that CPA could be involved in the metabolism of leukotrienesThe aim of this study was to clone the gene encoding the human mast cell carboxypeptidase A (hMC - CPA) in yeastThe results showed that the gene was expressed in Ecoli when cloned into the expression vector of Ecoli , pET28a



Cloning in yeast Human lung mast cell carboxypeptidase