015360000a22002290004500003000900000005001700009008004100026040002800067041000800095100003200103245019500135246018500330260005100515300002800566502008800594520047000682530002201152653002101174653004201195700003601237856003301273EG-GICUC20250223032127.0061016s2006    ua d   f m    000 0 eng d  aEG-GICUCbengcEG-GICUC0 aEng0 aMohammed Aref Marwan Kyyaly10aCloning , overexpression , and characterization of human lung mast cell carboxypeptidase a in the yeast saccharomyces cerevisiae / cMohammed Aref Marwan Kyyaly ; Supervised Sayed Kamel Goda15aاستنساخ والتعبير عن ودراسة انزيم الكربوكسى ببتيديز من خلايا الرئة البشرية الجذعية فى خميرة الخبز  aCairo : bMohammed Aref Marwan Kyyaly , c2006  a89P : bdiagrs ; c30cm  aThesis (M.Sc.) - Cairo University - Faculty Of Science - Department Of Biochemistry  aHuman skin and lung mast cells contain a carboxypeptidase in their secretory granulesThe biological function of carboxypeptidase A , CPA is uncertain but previous studies suggested that CPA could be involved in the metabolism of leukotrienesThe aim of this study was to clone the gene encoding the human mast cell carboxypeptidase A (hMC - CPA) in yeastThe results showed that the gene was expressed in Ecoli when cloned into the expression vector of Ecoli , pET28a  aIssued also as CD 4aCloning in yeast 4aHuman lung mast cell carboxypeptidase0 aSayed Kamel Goda , eSupervisor  uhttp://172.23.153.220/th.pdf