02526cam a2200265 a 4500003000900000005001700009008004100026040002800067041000800095100002700103245024500130246018700375260004600562300004400608502009700652520129600749530002202045653001202067653001602079653001102095700004002106700004502146700003602191856003302227EG-GiCUC20250223032222.0190307s2018    ua dh  f m    000 0 eng d  aEG-GiCUCbengcEG-GiCUC0 aeng0 aRehab Nagy Ibrahim Ali10aEffect of heat elevation on buffalo (bubalus bubalis) oocytes{u2019} competence with particular reference to related gene expression / cRehab Nagy Ibrahim Ali ; Supervised Amal Kamal Elasheeri , Ashraf Abdelhalim Elsayed , Laila Nasser Eid15aتأثير ارتفاع درجة الحرارة على كفاءة الخلايا البيضية للجاموس و علاقتها بتعبير الجينات المرتبطة بها  aCairo : bRehab Nagy Ibrahim Ali , c2018  a109 P. : bcharts , facsimiles ; c25cm  aThesis (Ph.D.) - Cairo University - Faculty of Agriculture - Department of Animal Production  aThis study was performed to investigate the effects of physiologically relevant heat shock during oocyte maturation on the developmental competence of Egyptian buffalo oocytes and the expression of heat stress genes. This study was designed into three experiments. The first and second experiments were run on three groups, (n=250 oocyte/group), buffalo cumulus oocyte complexes (COCs) were cultured at 38.5{u00B0} C (T0, control) or were exposed to 39.5{u00B0} C (T1) or 40.5{u00B0} C (T2) for the first 6 h of the in vitro maturation (IVM). After this period, all groups were incubated at 38.5{u00B0}C up to the blastocyst stage (7 day). In the 1st experiment, the groups were exposed to the assigned temperatures to study the effect of partial exposure of buffalo oocytes to heat stress (T1 and T2) on their maturation rate. The 2nd experiment has studied the ability of in vitro fertilized buffalo oocytes to develop until the blastocyst stage after being exposed to partial heat stress (T1 and T2). The 3rd experiment was conducted to draw the profile of mRNA expression of selected target genes (HSF-1, HSF-2, HSP-70, HSP-90, BAX, P53, SOD1, COX1, MAPK14) in denuded oocytes and their isolated cumulus cells resulted from control COCs as well as from COCs exposed to 39.5{u00B0} C (T1)  aIssued also as CD 4aBuffalo 4aHeat stress 4aOocyte0 aAmal Kamal Elasheeri , eSupervisor0 aAshraf Abdelhalim Elsayed , eSupervisor0 aLaila Nasser Eid , eSupervisor  uhttp://172.23.153.220/th.pdf