02540cam a2200265 a 4500003000900000005001700009008004100026040002800067041000800095100003100103245027100134246016300405260005000568300005700618502014600675520117800821530002201999653002102021653005202042653002002094700004302114700004602157700003802203856003302241EG-GiCUC20250223032229.0190319s2018    ua dho f m    000 0 eng d  aEG-GiCUCbengcEG-GiCUC0 aeng0 aTamer Mohsen Ahmed Elnahas10aRandomized prospective study on the use of laser assisted hatching for transfer of frozen / thawed embryos in human intracytoplasmic sperm injection / cTamer Mohsen Ahmed Elnahas ; Supervised Mohamed Amr Hussein Elnory , Mohamed Ahmed Aboulghar , Osama Mahmoud Azmy15aدراسة عشوائية محتملة لتأثير الليزر كمحفز لفقس الاجنة المجمدة فى حالات الحقن المجهرى  aCairo : bTamer Mohsen Ahmed Elnahas , c2018  a91 P. : bcharts , facsimiles , photographs ; c25cm  aThesis (Ph.D.) - Cairo University - National Institute of Laser Enhanced Sciences - Department of Laser Application in Medical and Biological  aBackground: Cryopreservation of human embryos has become an essential part of assisted reproduction, however it might be associated with zonal hardening, which could reduce implantation rates and clinical pregnancy rates. Assisted hatching has been used to induce microdissection of the zona pellucida. Many techniques have been used. Such as mechanical, chemical, piezo and laser techniques. Objective: To determine if laser assisted hatching improves both the implantation and clinical pregnancy rates in women using transferred frozen/thawed embryos. Patients and methods: 160 women. Scheduled for transfer of cryopreserved embryos, were randomized in two equal groups using sealed envelopes; a laser assisted hatching group (test group), and a control group, each included 80 women. On the day of embryo transfer. Diode laser was used in LAH group to induce zonal microdissection. The quality and safety of the embryos are assisted morphologically after hatching and also by measuring malondialdehyde before and after application of laser to determine if laser might be a source of oxidative stress, which affects embryos quality, implantation rates and pregnancy rates  aIssued also as CD 4aCryopreservation 4aLaser assisted hatching for transfer of frozen  4aPregnancy rates0 aMohamed Ahmed Aboulghar , eSupervisor0 aMohamed Amr Hussein Elnory , eSupervisor0 aOsama Mahmoud Azmy , eSupervisor  uhttp://172.23.153.220/th.pdf