020070000a22003370004500 EG-GICUC 20250223032358.0 061012s2006 ua a f m 000 0 eng d EG-GICUC eng EG-GICUC Eng Deposite M.Sc Cai01.12.02.M.Sc.2006.TaM. Tawfik Ghiyas Allbabidi Molecular studies on industrilly important , phenylalanine free casein gene in yeast / Tawfik Ghiyas Allbabidi ; Supervised Sayed Kamel Goda دراسات جزيئية صناعية هامة على الكازين الخالى من الفنيل النين فى الخميرة Cairo : Tawfik Ghiyas Allbabidi , 2006 74L : ill ; 30cm Thesis (M.Sc.) - Cairo University - Faculty Of Science - Department Of Biochemistry As a prelude to developing a yeast - based fermentation process for the production of phenylalanine free alpha - casein as a foodstuff for patients suffering from phenylketonuria , we cloned a gene encoding a nutritional protein (bovine alpha - casein) , but devoid of phenylalanine codons and with a codon bias similar to that of naturally occurring highly expressed genes in ScerevisiaeThe results show that synthetic gene is expressed in Ecoli when cloned into the Ecoli expression vector pET28a , and expressed in Scerevisiae when cloned into yeast expression vector pYES2 , Western blot analysis has confirmed the expression of the gene understudy conclusion : We cloned and expressed a gene devoid of any phenylalanine codon to produce a foodstuff for PKU patients Issued also as CD phenylalanine free protein Phenylketonuria PKU) expression in yeast synthetic gene expression Sayed Kamel Goda , Supervisor http://172.23.153.220/th.pdf Aml Cataloger Esam Revisor ddc TH 73888 73888 ddc 0 CUCL cl210 2024-02-11 Cai01.12.02.M.Sc.2006.TaM. 01010110046009000 2023-09-22 00:00:00 TH ddc 0 CUCL cl2u8 2024-02-11 Cai01.12.02.M.Sc.2006.TaM. 01020110046009000 2023-09-22 00:00:00 CD