TY  - GEN
AU  - Tawfik Ghiyas Allbabidi
AU  - Sayed Kamel Goda , 
TI  - Molecular studies on industrilly important , phenylalanine free casein gene in yeast / 
PY  - 2006///
CY  - Cairo : 
PB  - Tawfik Ghiyas Allbabidi , 
KW  - phenylalanine free protein
KW  - Phenylketonuria
KW  - PKU) expression in yeast
KW  - synthetic gene expression
N1  - Thesis (M.Sc.) - Cairo University - Faculty Of Science - Department Of Biochemistry; Issued also as CD
N2  - As a prelude to developing a yeast - based fermentation process for the production of phenylalanine free alpha - casein as a foodstuff for patients suffering from phenylketonuria , we cloned a gene encoding a nutritional protein (bovine alpha - casein) , but devoid of phenylalanine codons and with a codon bias similar to that of naturally occurring highly expressed genes in ScerevisiaeThe results show that synthetic gene is expressed in Ecoli when cloned into the Ecoli expression vector pET28a , and expressed in Scerevisiae when cloned into yeast expression vector pYES2 , Western blot analysis has confirmed the expression of the gene understudy conclusion : We cloned and expressed a gene devoid of any phenylalanine codon to produce a foodstuff for PKU patients
UR  - http://172.23.153.220/th.pdf
ER  -