Microbiological studies on ESÝL pseudomonas spp. recovered from raw camel meat /
دراسات ميكروبيولوجية على ميكروب السودوموناس المفرز لأنزيم البيتالاكتاميز واسع المدى و المعزول من لحوم الأبل
Amr Hassan Mahran ; Supervised Kamelia Mahmoud Osman , Ahmed Orabi Hassan
- Cairo : Amr Hassan Mahran Ali , 2019
- 64 P. ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Veterinary Medicine - Department of Microbiology
The present investigation was the first endeavor to elucidate the strain identification and QS-dependent virulence, biofilm formation and antibiotic resistance characterization of the one humped camel) retail meat-derived Pseudomonas species. Pseudomonas could aggravate the problem of multidrug-resistant as a high prevalence of resistance to sulfamethoxazole/trimethoprim and ciprofloxacin was observed. All strains were resistant to tetracycline, ampicillin and nalidixic acid and susceptible to amikacin, imipenem, gentamicin, cefepime and ceftriaxone. Pseudomonas isolates were able to produce . Two out of the eight pseudomonas aeruginosa isolates and one of the two Pseudomonas fluorescens were deficient in the five quorum-sensing (QS) genes(lasI, lasR, rhlR, rhlAB and rhlI). One of the two pseudomonas fluorescens carried three out of the five QS genes. The rhlAB gene was present in 10% of the isolates, while lasI gene was present in 20% of the isolates., also lasI, rhlR and rhlI genes in 10% of the isolates., however lasR, rhlR and rhlI in 10%., in the other hand lasI, rhlAB and rhlI in 20%., finally lasI, rhlAB and rhlI in 10% of the isolates. Distribution of the virulence genes toxA, aprA and plcH as follow; Two of the Pseudomonas isolates did not carry any of the three virulence genes., while the virulence gene toxA was evident in two of the Pseudomonas isolates