Warda Alaa Eldin Mahmoud Hashim <br/><br/>
Isolation, molecular characterization and expression of nitrite reductase (NiR) gene responsible for the absorption of some air pollutants /  
 عزل و توصيف جزيئى والتعبير عن جين مختزل النيتريت المسؤول عن امتصاص بعض ملوثات الهواء 
Warda Alaa Eldin Mahmoud Hashim ; Supervised Said Abdrahman Salih , Rania Mahmoud Abou Ali 
 - Cairo :   Warda Alaa Eldin Mahmoud Hashim ,   2020 
 - 86 P. :   charts , facsimiles ;   25cm 
<br/><br/>Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology 
<br/><br/> A nitrite reductase (NiR) gene was isolated, cloned and sequenced from nitrate induced leaves of the Egyptian Spinacia oleracea seedlings. The sequence of the isolated NiR gene has 1788 bp open reading frame. The NiR sequence was submitted into GenBank under accession number MH729808. The deduced amino acid sequence is 595 a.a, it has 96% homology with predicted ferredoxin-nitrite reductase, chloroplastic (Spinacia oleracea) and highly conserved. Leucine was the highest amino acid percentage in the predicted NiR protein, which has a higher preference for the Ü-helix. RT-PCR analysis showed that the expression level of NiR was the highest at 2 hr of nitrate treatment. The NiR protein had high activity towards NaNO2 substrate after 3 hr induction with potassium nitrate 
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<br/><br/><label>Subjects--Index Terms: </label>Nitrate treatment  Nitrite reductase (NiR) activity  Real Time-PCR