02185cam a2200337 a 4500 EG-GiCUC 20250223032525.0 200220s2020 ua dh f m 000 0 eng d EG-GiCUC eng EG-GiCUC eng Deposite M.Sc Cai01.12.25.M.Sc.2020.Wa.I Warda Alaa Eldin Mahmoud Hashim Isolation, molecular characterization and expression of nitrite reductase (NiR) gene responsible for the absorption of some air pollutants / Warda Alaa Eldin Mahmoud Hashim ; Supervised Said Abdrahman Salih , Rania Mahmoud Abou Ali عزل و توصيف جزيئى والتعبير عن جين مختزل النيتريت المسؤول عن امتصاص بعض ملوثات الهواء Cairo : Warda Alaa Eldin Mahmoud Hashim , 2020 86 P. : charts , facsimiles ; 25cm Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology A nitrite reductase (NiR) gene was isolated, cloned and sequenced from nitrate induced leaves of the Egyptian Spinacia oleracea seedlings. The sequence of the isolated NiR gene has 1788 bp open reading frame. The NiR sequence was submitted into GenBank under accession number MH729808. The deduced amino acid sequence is 595 a.a, it has 96% homology with predicted ferredoxin-nitrite reductase, chloroplastic (Spinacia oleracea) and highly conserved. Leucine was the highest amino acid percentage in the predicted NiR protein, which has a higher preference for the Ü-helix. RT-PCR analysis showed that the expression level of NiR was the highest at 2 hr of nitrate treatment. The NiR protein had high activity towards NaNO2 substrate after 3 hr induction with potassium nitrate Issued also as CD Nitrate treatment Nitrite reductase (NiR) activity Real Time-PCR Rania Mahmoud Abou Ali , Supervisor Said Abdrahman Salih , Supervisor http://172.23.153.220/th.pdf Asmaa Cataloger Nazla Revisor ddc TH 76717 76717 ddc 0 CUCL cl210 2024-02-11 Cai01.12.25.M.Sc.2020.Wa.I 01010110080864000 2023-09-22 00:00:00 TH ddc 0 CUCL cl2u8 2024-02-11 Cai01.12.25.M.Sc.2020.Wa.I 01020110080864000 2023-09-22 00:00:00 80864.CD CD