03220cam a2200265 a 4500003000900000005001700009008004100026040002800067041000800095100003600103245030200139246030400441260005500745300004400800502011200844520173100956530002202687653003702709653003002746653002502776700004302801700003802844700003902882856003302921EG-GiCUC20250223032528.0200224s2019    ua do  f m    000 0 eng d  aEG-GiCUCbengcEG-GiCUC0 aeng0 aLaila Akmal Emad Eldien Elokaly10aMicrobiological assessment after chemo-mechanical caries removal using papain-based enzyme versus conventional rotary tools in occlusal carious lesions : bRandomized clinical trial / cLaila Akmal Emad Eldien Elokaly ; Supervised Mai Mahmoud Yousry , Essam Abdelhafez Naguib , Mai Mamdouh Mohamed15aالتقييم الميكروبيولوجى بعد الإزالة الكيميائية للتسوس باستخدام إنزيم بابين مقابل الأدوات الدوارة المعتادة في آفات التسوس الطبقى : bدراسة إكلينيكية عشوائية  aCairo : bLaila Akmal Emad Eldien Elokaly , c2019  a80 P. : bcharts , photographs ; c25cm  aThesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Operative Dentistry  aThe study was performed to assess the efficacy of caries excavation using the papain-based chemo-mechanical method (Brix 3000) in comparison to conventional rotary tools in the reduction of Streptococcus Mutans count in Occlusal carious cavities. Materials and methods: Double-blinded, two-armed, split-mouth and a randomized clinical trial was conducted in the clinic of the Conservative Dentistry Department, Faculty of Dentistry, Cairo University, Egypt. Twenty-three patients aged between 18-40 years with active carious lesions in a permanent lower molar (each patient had at least two cavitied molars) were randomly allocated into two groups: a test group (N=23): Brix3000 and a Control group (N=23): Conventional rotary. From each group, two dentin samples were collected before and after caries removal. In both groups, the central cariogenic biomass and superficial parts of the necrotic dentin was removed with the excavator and then discarded. Then, a dentin sample was collected using a sharp, sterile excavator. And then the dentin samples were immediately transferred to the sterile disposable test tube containing 1.5 ml thioglycollate medium used as a carrier and kept in an icebox then taken to the microbiology laboratory for processing within two hours. The sterilized bottles containing the dentin samples were shaken in a vortex for the 30s to disperse bacterial aggregates and decimal dilutions were then prepared in sterile saline (0.9% NaCl). Next, 50 ol aliquots of each dilution were spread using micropipette onto the following solid media and spread on the surface of the agar using a sterile glass rod to give homogenous bacterial growth. Mitis salivarius agar (MSA) was used for Streptococcus spp  aIssued also as CD 4aChemo-mechanical caries removal  4aOcclusal carious lesions  4aPapain-based enzyme 0 aEssam Abdelhafez Naguib , eSupervisor0 aMai Mahmoud Yousry , eSupervisor0 aMai Mamdouh Mohamed , eSupervisor  uhttp://172.23.153.220/th.pdf