03083cam a2200349 a 4500 EG-GiCUC 20250223032554.0 200908s2020 ua dh f m 000 0 eng d EG-GiCUC eng EG-GiCUC eng Deposite M.Sc Cai01.12.25.M.Sc.2020.Is.S Ismail Mohamed Rafeky Hussien Saad Studying HCV IRES sequence and structure for genotype 4a-in correlation to interferon ribavirin responding treatment in Egyptian patients using bioinformatics / Ismail Mohamed Rafeky Hussien Saad ; Supertvised Fawzy Ali Ataby , Salwa Farouk Sabet , Mohamed Ali Saber دراسة المنطقه المسؤله عن الدخول الي الريبوسوم الداخلى فى فيروس التهاب الكبدى سى من حيث التسلسل النيكولتيدى و التركيب الثانوى للسلاله ( 4أ) و علاقه ذلك بالاستجابه لعلاج الانترفيرون\ريبافيرين فى حالات المرضى المصرين باستخدام المعلوماتية الحيوية Cairo : Ismail Mohamed Rafeky Hussien Saad , 2020 113 P. : charts , facsimiles ; 25cm Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology Hepatitis C virus infection is the major cause of chronic hepatitis. The HCV genotype 4a is predominant in Egyptian patients that are not responding to the combined pegylated interferon-alpha /ribavirin therapy. The HCV IRES is a well-defined structure of about 341 nucleotides in its 5{u00B4}-untranslated region (UTR). This study was conducted on 46 Quantification of HCV-RNA in serum has been carried out at the beginning of treatment (W0) and at week12 (W12), patients 26 responders and 20 non-responders to Study HCV IRES Sequence and Secondary Structure and find the Correlation to pegylated Interferon\Ribavirin Responding Treatment in Egyptian patients. There was no statistically significant difference between Responders and Non-Responders regarding gender (P=0.1), age (p=0.3), AFP (p=0.4) and Albumin (p=0.1) while There was a significant increase for AST/ALT (p=0.002, p=0.001) respectively. HCV IRES RT-PCR and sequence analysis including genotyping, MSA and Sequence variation showed that 98% genotype 4a and there was no significant difference in sequence between Responders and Non-Responders patients in addition to the prediction of HCV IRES secondary structure MFE,Centroid revealed that there was no effect on IRES secondary structure and it is conserved among all HCV genotypes Issued also as CD Genotype 4a Hepatitis C Virus (HCV) Polymerase Chain Reaction (qPCR) Fawzy Ali Ataby , Supervisor Mohamed Ali Saber , Supervisor Salwa Farouk Sabet , Supervisor http://172.23.153.220/th.pdf Nazla Revisor Shimaa Cataloger ddc TH 77636 77636 ddc 0 CUCL cl210 2024-02-11 Cai01.12.25.M.Sc.2020.Is.S 01010110081454000 2023-09-22 00:00:00 TH ddc 0 CUCL cl2u8 2024-02-11 Cai01.12.25.M.Sc.2020.Is.S 01020110081454000 2023-09-22 00:00:00 81454.CD CD