Regulation of doxorubicin chemotherapeutic potential by MiR-520c-3p in hepatocellular carcinoma cells /
فى خلايا سرطان الكبد (MiR-520c-3p)تنظيم الامكانية العلاجية للدكسوروبيسين عن طريق الحمض الميكروريبوزى النووى
Mohamed Attia Ragheb Erfan ; Supervised Mervat Elsayed Mohamed , Emad Mahmoud Ibrahim Elzayat , Abdelhady Ali Abdelwahab
- Cairo : Mohamed Attia Ragheb Erfan , 2020
- 160 P . : charts , facsmilies ; 25cm
Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry
Background: Doxorubicin (Dox) is one of the most common drugs used in cancer therapy, including hepatocellular carcinoma (HCC). Drug resistance, is one of chemotherapys significant problems. Emerging studies have shown that microRNAs (miRNAs) could participate in regulating this mechanism. Nevertheless, the impact of miRNAs on HCC chemoresistance is still enigmatic. Objective: Investigating the role of a candidate miRNA in enhancement of anti-tumor effects of Dox against HepG2 cells. Expression profile for liver related miRNAs (384 miRNAs) has been analyzed on HepG2 cells treated with Dox using qRT-PCR. miR- 520c-3p, the most deregulated miRNA, was selected for combination treatment with Dox. Methods: Expression level for LEF1, CDK2, CDH1, VIM, Mcl-1 and p53 was evaluated in miR-520c-3p transfected cells. Cell viability, colony formation, wound healing as well as apoptosis assays have been demonstrated. Furthermore, Mcl-1 protein level was measured using a western blot technique. Results: The present data indicates that miR-520c-3p overexpression could render HepG2 cells chemo-sensitive to Dox through enhancing its suppressive effects on proliferation, migration, and induction of apoptosis