02880cam a2200349 a 4500 EG-GiCUC 20250223032649.0 201231s2020 ua dho f m 000 0 eng d EG-GiCUC eng EG-GiCUC eng Deposite M.Sc Cai01.12.25.M.Sc.2020.No.C Norhan Yasser Sayed Moaz Characterization and functional analysis of an insecticidal protein from baculovirus / Norhan Yasser Sayed Moaz ; Supervised Saeed Ahmed Soliman Ghozlan , Maha Mohamed Youssef Alkhazindar , Wael Hassan Ali Elmenofy التوصيف والتحليل الوظيفي لبروتين مبيد للحشرات من الفيروس العصوي Cairo : Norhan Yasser Sayed Moaz , 2020 61 P . , (12) P. : charts , facsmilies , photographs ; 25cm Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Biotechnology Chitinase plays a highly important function in the growth of insects and its development by hydrolyzing chitin in the body of the insect. In this study we have cloned, expressed and characterized chitinase gene A (ChiA) from the cotton leafworm, Spodoptera littoralis nucleopolyhedrovirus, an Egyptian isolate. The Spli-ChiA gene shown to have an open reading frame of 1809 nucleotides, encoding a protein of 599 amino acid residues with a predicted weight of 66.8 kDa. The phylogenetic analysis revealed that the Spli-ChiA deduced protein sequence shared extensive similarities with other baculovirus chitinases to be found in the GenBank. The SDS-PAGE analysis of SpliNPV-infected larvae total protein followed by western blotting with anti-SpliNPV chitinase antibody identified a protein of about ~50 kDa corresponding to the mature chitinase of SpliNPV. Temporal transcriptional analyses using RT-PCR and qRT-PCR revealed that Spli-ChiA transcription might be detected in SpliNPV infect larvae at 12 hpi with a steady increase in the transcription levels up to 71.4-fold at 120 hpi, suggesting the late transcription of chitinase gene. In addition, the purified Spli-ChiA protein showed inhibition in vitro of the growth of two fungi species, Fusarium solani and Fusarium oxysporum, confirming the expressed protein activity. The results concluded that SpliNPV chitinase (Spli-ChiA) is a good applicant protein for significant contribution in pest control Issued also as CD Chitinase gene A qRT-PCR Spodoptera littoralis NPV Maha Mohamed Youssef Alkhazindar , Supervisor Said Ahmed Soliman Ghozlan , Supervisor Wael Hassan Ali Elmenofy , Supervisor http://172.23.153.220/th.pdf Amira Cataloger Nazla Revisor ddc TH 79396 79396 ddc 0 CUCL cl210 2024-02-11 Cai01.12.25.M.Sc.2020.No.C 01010110082455000 2023-09-22 00:00:00 TH ddc 0 CUCL cl2u8 2024-02-11 Cai01.12.25.M.Sc.2020.No.C 01020110082455000 2023-09-22 00:00:00 82455.CD CD