02696cam a2200265 a 4500003000900000005001700009008004100026040002800067041000800095100003400103245022100137246019100358260005300549300003200602502008800634520146400722530002202186653001802208653002002226653002102246700004302267700004202310700004502352856003302397EG-GiCUC20250223032745.0210605s2021    ua d   f m    000 0 eng d  aEG-GiCUCbengcEG-GiCUC0 aeng0 aHadeer Hesham Abdelfattah Aly10aCirculating MicroRNAs as a prognostic biomarker predicting clinical outcome in breast cancer / cHadeer Hesham Abdelfattah Aly ; Supervised Hamed Abdelfatah Dabaon , Mohamed Aly Eldessouky , Neemat Mohamed Aly Kassem15aالاحماض الميكرونووية ريبوزية الدوارة كعلامة بيولوجية نذيرة للتنبؤ بالنتيجة السريرية فى سرطان الثدى  aCairo : bHadeer Hesham Abdelfattah Aly , c2021  a112 P. : bcharts ; c25cm   aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry  aBreast cancer is one of the most common cancers in women worldwide. Over 1.5 million women (25% of all women with cancer) are diagnosed with breast cancer every year throughout the world. Purpose & Methodology Quantitative assessment of plasma miRNA-34a & miRNA-125b expression was performed by qRT-PCR to predict outcome from neoadjuvant chemotherapy. Thirty nine newly diagnosed locally advanced breast cancer female patients with 10 age and sex matched healthy volunteers were included in the study. Results We performed ROC curve analysis to evaluate the diagnostic value for the miRNA-34a with AUCs = 0.995, cutoff point of 2.57 sensitivity 97.4%, specificity 100%, PPV 100%, NPV 83.3% and accuracy 97.7%.miRNA-125b had AUC = 0.68 and a cutoff point of 8.69 with sensitivity 66.7%, specificity 70.0%, PPV 90.6%, NPV 41.2% and accuracy 73.5%. miRNA-34a expression were significantly higher in breast cancer patients compared to controls with p value <0.001*. Also, miRNA-34a expression level was significantly higher in patients with progressive disease with P value =0.03*. However, miRNA-125b expression levels were insignificantly higher in responsive patients with p value = 0.2. Conclusion miRNAs are crucial candidates for novel molecular targeted therapies due to their capability to regulate numerous genes in molecular pathways. miRNA-34a can predict chemotherapeutic resistance associated with higher expression levels in non-responsive patients  aIssued also as CD 4aBreast cancer 4aDNA technology  4aGene expression 0 aHamed Abdelfatah Dabaon , eSupervisor0 aMohamed Aly Eldessouky , eSupervisor0 aNeemat Mohamed Aly Kassem , eSupervisor  uhttp://172.23.153.220/th.pdf