02795cam a2200349 a 4500 EG-GiCUC 20250223032747.0 210612s2021 ua dh f m 000 0 eng d EG-GiCUC eng EG-GiCUC eng Deposite Ph.D Cai01.12.02.Ph.D.2021.Na.S Nada Sabry Ibraheem Abdelhady Synthesis and biological evaluation of novel chalcones as anticancer agents / Nada Sabry Ibraheem Abdelhady ; Supervised Ismail Abdelshafy Abdelhamid , Magda Fikry Mahmoud Mohamed , Salwa Mohamed Elhallouty تشييد وتقييم بيولوجى لشالكونات جديده كمضادات للسرطان Cairo : Nada Sabry Ibraheem Abdelhady , 2021 144 P. : charts , facsimiles ; 25cm Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry Two novel chemotherapeutic chalcones were synthesized and their structures were confirmed by different spectral tools.Theoretical studies such as molecular modeling were done to detect the mechanism of action of these compounds. In vitro cytotoxicity showed a strong effect against all tested cell lines (MCF7, A459, HepG2, and HCT116), and low toxic effect against normal human melanocytes (HFB4).The lung carcinoma cell line was chosen for further molecular studies. Real-time PCR demonstrated that the two compounds upregulated gene expression of (BAX, p53, casp-3, casp-8, casp-9) genes and decreased the expression of anti-apoptotic genes bcl2, CDK4, and MMP1.Flow-cytometry indicated that cell cycle arrest of A459 was induced at the G2/M phase and the apoptotic percentage increased significantly compared to the control sample. Cytochrome c oxidase and VEGF enzyme activity were detected by ELISA assay. SEM tool was used to follow the morphological changes that occurred on the cell surface, cell granulation, and average roughness of the cell surface. The change in the number and morphology of mitochondria, cell shrinkage, increase in the number of cytoplasmic organelles, membrane blebbing, chromatin condensation, and apoptotic bodies were observed using TEM. The obtained data suggested that new chalcones exerted their pathways on lung carcinoma through induction of two pathways of apoptosis Issued also as CD [l, 2, 4]Triazolo[3,4-a]isoquinoline Chalcones Cytotoxicity Ismail Abdelshafy Abdelhamid , Supervisor Magda Fikry Mahmoud Mohamed , Supervisor Salwa Mohamed Elhallouty , Supervisor http://172.23.153.220/th.pdf Nazla Revisor Shimaa Cataloger ddc TH 81253 81253 ddc 0 CUCL cl210 2024-02-11 Cai01.12.02.Ph.D.2021.Na.S 01010110083618000 2023-09-22 00:00:00 TH ddc 0 CUCL cl2u8 2024-02-11 Cai01.12.02.Ph.D.2021.Na.S 01020110083618000 2023-09-22 00:00:00 83618.CD CD