TY  - BOOK
AU  - Taher Talal Mahrous Ahmed
AU  - Mohamed G. Farahat , 
AU  - Zienat Kamel Mohamed Ibrahim , 
TI  - Cloning, expression and characterization of bacterial L - glutaminase / 
PY  - 2021///
CY  - Cairo : 
PB  - Taher Talal Mahrous Ahmed , 
KW  -  L-Glutaminase
KW  - Cloning
KW  - Screening
N1  - Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany - Microbiology; Issued also as CD
N2  - L-Glutaminase has received an increased importance in recent years for its anti-carcinogenic potential. In this study, Klebsiella michiganensis was isolated from the Lake Mariout and the glsA gene encoding L-glutaminase from was cloned into pQE-80L expression vector and expressed in E. coli BL21.The recombinant histidine-tagged L-glutaminasewas purified to homogeneity by Ni{u2013}NTAaffinity chromatography and displayed a single 33.0 kDa band on SDS-PAGE.The purified functional enzyme exhibited a specific activity at 89 U/mg and inhibited the growth of human colon cancer cells (HCT116) with IC50 value of 2.4±0.7 U/ml
UR  - http://172.23.153.220/th.pdf
ER  -