03481cam a2200349 a 4500 EG-GiCUC 20250223032844.0 211108s2021 ua dh f m 000 0 eng d EG-GiCUC eng EG-GiCUC eng Deposite Ph.D Cai01.12.02.Ph.D.2021.Ay.B Aya Abdelaziz Hanafey Mahmoud Biochemical and molecular effects of some plant extracts on streptozotocin induced diabetic rats / Aya Abdelaziz Hanafey Mahmoud ; Supervised Mohamed Ali Eldesouky , Doaa Samir Salah Eldin , Khairy Abdelmoneim Ibrahim التأثيرات الكيميائية الحيوية والجزيئية لبعض مستخلصات النباتات على الجرذان المصابة بمرض السكرى المستحث بالستربتوزوتوسين Cairo : Aya Abdelaziz Hanafey Mahmoud , 2021 144 P. : charts , facsimiles ; 25cm Thesis (Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry Study objective: The aim of the study is to assess the effect of aqueous extract of Pterocarpus santalinus heart wood and Brassica nigra seeds on amelioration of type 2 diabetes mellitus and evaluate the roles of these extracts on recover of diabetic nephropathy and diabetic liver injury in streptozotocin induced diabetic rats. The study is include 24 adult male albino rats and were divided into four groups, each group contain 6 animals. Group 1 served as control group. For induction of diabetes rats were received STZ (65 mg/kg body weight). After STZ induction the remaining three groups were split as follow: group 2 served as diabetic untreated group, group 3 served as diabetic group treated with aqueous Pterocarpus santalinus extract and group 4 served as diabetic group treated with aqueous Brassica nigra extract. Results: STZ administration produced significant up-regulation in the mRNA of Thioredoxininteracting protein (TXNIP), The Nod-like receptor protein 3(NLRP3) inflammasome and Apoptosis-enhancing nuclease (AEN). Also STZ administration produced significant increase in serum glucose, HbA1c, Homeostatic Model Assessment insulin resistance (HOMA-IR), lipids, creatinine, urea, ALT and AST levels; also, significant decrease in insulin, Homeostatic Model Assessment insulin sensitivity (HOMA-IS), Quantitative Insulin Sensitivity Check Index (QUICKI) and HDL levels. The effects of aqueous extracts of pterocarpus santalinus wood and Brassica nigra seeds were significant down-regulated the mRNA of TXNIP, NLRP3 and AEN. Also the results of this study showed that after treatment by aqueous of Pterocarpus santalinus wood and Brassica nigra seeds there were significant decreased in serum levels of glucose, HbA1c, lipids, creatinine, urea, ALT and AST as well as significant increase in insulin, Homeostatic Model Assessment insulin sensitivity (HOMA-IS), Quantitative Insulin Sensitivity Check Index (QUICKI) and HDL levels Issued also as CD Brassica nigra seeds Pterocarpus santalinus heart wood Type 2 diabetes mellitus Doaa Samir Salah Eldin , Supervisor Khairy Abdelmoneim Ibrahim , Supervisor Mohamed Ali Eldesouky , Supervisor http://172.23.153.220/th.pdf Nazla Revisor Shimaa Cataloger ddc TH 83043 83043 ddc 0 CUCL cl210 2024-02-11 Cai01.12.02.Ph.D.2021.Ay.B 01010110084664000 2023-09-22 00:00:00 TH ddc 0 CUCL cl2u8 2024-02-11 Cai01.12.02.Ph.D.2021.Ay.B 01020110084664000 2023-09-22 00:00:00 84664.CD CD