03267cam a2200301 a 4500003000900000005001700009008004100026040002800067041001800095082000800113100005800121245017800179246007100357264000900428300005000437336002200487337002500509338002300534502009500557504003300652520206800685530002202753546005402775650001702829653003602846700004002882700004302922EG-GiCUC20260517111229.0220305s2022    ua dh  frm    000 0 eng d  aEG-GiCUCbengcEG-GiCUC0 aengbengbara04a5800 aHanan Mohammed Kamal Abdelfatah Osman, epreparation.10aProduction and characterization of microbial myrosinase / cby Hanan Mohammed Kamal Abdelfatah Osman ; Supervised Prof.Dr. Mary Sobhy Khalil, Prof.Dr. Sameh Heikal Youseif. 15aانتاج وتوصيف الميروسينيز الميكروبى  0c2022  a162 P. : bcharts , facsimiles ; c25cm+ eCD  atext2rda content  aUnmediated2rdamedia  avolume2rdacarrier  aThesis (Ph.D.) - Cairo University - Faculty of Science - Department of Botany Microbiology  aBibliography: Pages 191-228.  aMyrosinase hydrolyzes glucosinolates giving various products depending on the physiological conditions.The hydrolysis products especially isothiocyanates have antibacterial, antifungal, anticancer activity, act as soil biofumigant, natural pesticides  and food preservatives. Myrosinase is mainly found in cruciferous plants but few  literatures reported its production by both fungi and bacteria. In this study, 94 microbial  isolates from E.sativa and its soil rhizosphere were screened for myrosinase, 25% of  the screened bacterial isolates (9 isolates) and 62.07% of the screened fungal isolates (36 isolates) had myrosinase activity. About 22.22% of the endophytic bacterial isolates from E.sativa roots and 63.63% of the endophytic bacterial isolates from E.sativa  leaves were able to produce myrosinase but none of soil rhizosphere and epiphytic bacterial isolates were able. On the other hand, 72.41% of the E.sativa fungal isolates  of soil rhizosphere, 50% of the endophytic fungal isolates from E.sativa roots, 37.5% of the endophytic fungal isolates from E.sativa leaves and 63.64% of the epiphytic isolates had myrosinase activity.This indicated that both endophytic bacterial and fungal population of E.sativa leaves and roots are good reservoir for myrosinase activity and the fungal community of the soil rhizosphere and the plant surfaces of E.sativa seemed to have myrosinase activity rather than the bacteria living there. The  nine myrosinase producing bacterial isolates were phenotypically characterized and  identified based on 16S rRNA sequencing.They all belonged to the genus Bacillus and  they were given the following accession numbers LC589981, LC589982, LC589983,  LC589984, LC589985, LC589986, LC589987, LC589988 and LC589989 on NCBI.  They were all most closely related to Bacillus siamensis and Bacillus velezensis with  identity percent > 99%. The fungal myrosinase producers were identified  morphologically and they belonged to six different genera: Aspergillus, Penicellium,  Fusarium, Eumericella, Alternaria and Sclerotium  aIssued also as CD  aText in English and abstract in Arabic & English. 0aMicrobiology 1aEruca sativaaFungiaMyrosinase0 aMary Sobhy Khalil ethesis advisor.0 aSameh Heikal Youseif ethesis advisor.