03371nam a2200421 a 4500 EG-GiCUC 20260516114337.0 220306s2021 ua dh frm 000 0 eng d EG-GiCUC eng EG-GiCUC eng eng ara Deposit 540 540 21 Ph.D Cai01.12.02.Ph.D.2021.Ma.E. Mahmod Morad Ragab Mohammed, preparation. Expression and polymorphism of long non-coding RNAs in colorectal cancer / Mahmod Morad Ragab Mohammed ; Supervised Prof.Dr. Ismail Abdelshafy Abdelhamid , Prof.Dr.Heba Mohammed Kamal Abdelhakeem , Prof.Dr.Olfat Gamil Shaker. التعبير والتباين الجينى للأحماض النووية الريبوزية الطويبة غير المشفرة فى سرطان القولون والمستقيم 2021 84 Pages : charts , facsimiles ; 25cm+ CD text rda content Unmediated rdamedia volume rdacarrier Thesis ( Ph.D.) - Cairo University - Faculty of Science - Department of Biochemistry Bibliography: pages 115-137. Background: Colorectal cancer (CRC) is a complex disease that develops as a consequence of both genetic and environmental risk factors. Diet, smoking and drinking habits are among environmental factors frequently associated with CRC risk. Aim of work:The present study aims to conduct a clinical biochemical study that aids in the investigation of some non-coding RNA expressions and polymorphisms (including long non-coding RNAs and miRNAs) namely, Plasmacytoma Variant Translocation-1 (PVT-1) and miR-186 in an attempt to provide new noninvasive diagnostic biomarkers for CRC of the Egyptian patients. Patients and methods: Eighty CRC studies and thirty healthy controls were included. Laboratory and pathological investigations were assessed. Serum miR-186 and long non-coding PVT-1 was measured as well as genotype for PVT-1 rs13255292. Results: The CRC patients had a mean age of 50.91±12.0. The mean serum miR-186 level in CRC patients (1.38±0.21) was significantly higher than in the control (0.93±0.1) (p=0.0001). The PVT-1 level in CRC patients was (5.91±0.25) significantly higher than control (1.1±0.2) (p=0.001). The PVT-1/ rs13255292 genotype CT was significantly higher compared to the control group. The T allele was highly significant with a p-value of 0.008 in the CRC patient group with respect to the control. Conclusion: miR-186, long noncoding PVT-1 as well as PVT-1 the T allele may be considered as genetic markers for CRC susceptibility Issued also as CD Text in English and abstract in Arabic & English. Biochemistry Colorectal Cancer (CRC) Long non-coding RNAs (lncRNAs) MicroRNAs (miRNAs) Heba Mohammed Kamal Abdelhakeem thesis advisor. Ismail Abd Elshafy Abd Elhamid thesis advisor. Olfat Gamil Shaker thesis advisor. 01-01-2022 Ismail Abdelshafy Abdelhamid Heba Mohammed Kamal Abdelhakeem Olfat Gamil Shaker Cairo University Faculty of Science Department of Biochemistry Shimaa Cataloger ddc TH 84414 ddc 148480 CUCL cl210 2024-02-11 85489 Cai01.12.02.Ph.D.2021.Ma.E. 01010110085489000 2023-09-22 00:00:00 2023-09-22 TH ddc 148481 CUCL cl2u8 2024-02-11 13824752 Cai01.12.02.Ph.D.2021.Ma.E. 01020110085489000 2023-09-22 00:00:00 85489.CD 2023-09-22 CD