03969nam a2200421 a 4500 EG-GiCUC 20260524130557.0 220807s2022 ua dh frm 000 0 eng d EG-GiCUC eng EG-GiCUC eng eng ara Deposite 632.9517 632.9517 21 Ph.D Cai01.16.03.Ph.D.2022.He.E Heba Moustafa Rasheed Hathout. preparation. Evaluation of the genotoxicity and oxidative stress of acetamiprid insecticide on nile tilapia : Oreochromis niloticus / Heba Moustafa Rasheed Hathout; Supervised Prof. Dr. Hassan. M. Sobhy تقييم السمية الجينية والجهد التأكسدي للمبيد الحشري اسيتامبريد على البلطي النيلي 2022 123p. : charts, facsimiles ; 25cm. CD text rda content Unmediated rdamedia volume rdacarrier Thesis (Ph.D.) - Cairo University - Faculty of African Postgraduate Studies - Department of Natural Resources Bibliography: p. 123-98. Acetamiprid (Aceta) is the most extensively used neonicotinoid which contaminate aquatic ecosystem from agricultural leaching. On juveniles of Oreochromis niloticus, the protective potential of ascorbic acid (Asc) against oxidative stress and genotoxicity induced by Aceta sub-lethal concentrations was investigated in this study. Fishes were divided into six groups and exposed to either; Asc (50 ppm), 10 ppm of Aceta, 20 ppm of Aceta, 10 ppm of Aceta and Asc (50 ppm), 20 ppm of Aceta and Asc (50 ppm). Unexposed animals were considered as control group. The experiment was conducted by the assessment of superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GPX) activities and their mRNA gene expression. DNA damage using comet assay in erythrocytes, hepatocytes and gill cells, in addition to mitotic index (MI), the existence of micronuclei (MN) and erythrocytic nuclear abnormalities (ENAs) were performed. The results showed that concentrations of Aceta (10 & 20 ppm) induced oxidative stress by decreasing the antioxidant enzyme activities and down-regulating gene expression of SOD, CAT and GPX compared to control group. However, LPO and NO levels were significantly elevated. Co-supplemented fish with Asc showed significant alleviations of oxidative status represented by substantial increases in the activities of SOD, CAT and GPX enzymes and diminished LPO and NO levels (P<0.05), along with a concomitant up-regulation of SOD and CAT gene expression (P < 0.05). There were genotoxic effects of Aceta exposure showed by the significant (P < 0.05) increase in DNA-damaged cells, MN, and ENA, meanwhile a decrease in MI. Supplementation with Asc reversed these effects in combined-exposure groups. Thus, results suggest that Aceta induced oxidative stress accompanied with genotoxicity. Further, Asc-combined exposure could be the effective treatment against Aceta-induced damage in O. niloticus. أعدت هذه الدراسة لفحص القدرة الوقائية لحمض الأسكوربيك ضد الجهد التأكسدي والسمية الجينية المسببة بواسطة جرعات المبيد الحشري (إسيتامبريد على صغار أسماك البلطي النيلي. Issued also as CD Text in English and abstract in Arabic & English. Genotoxicity Acetamiprid Insecticide Nile Tilapia Oxidative Stress Hassan. M. Sobhy thesis advisor. Islam M. El-Garawani thesis advisor. 01-01-2022 Hassan. M. Sobhy Cairo University Faculty of African Postgraduate Studies Department of Natural Resources Shimaa ddc TH 84718 ddc 149080 CUCL cl2u8 2024-02-11 13833091 Cai01.16.03.Ph.D.2022.He.E 01020110085656000 2023-09-22 00:00:00 85656.CD 2023-09-22 CD ddc 152410 CUCL cl210 2024-02-11 85656 Cai01.16.03.Ph.D.2022.He.E 01010110085656000 2023-09-22 00:00:00 2023-09-22 TH