Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

Objective: To study the in{uFB02}uence of female obesity on follicular {uFB02}uid oxidative stress and to correlate it to intracytoplasmic sperm injection (ICSI) outcome. Study design: Seventy-four normal females below the age of 40 undergoing ICSI for reason of male factor infertility were enrolled in the study. They were divided into 2 groups according to body mass index (BMI); Group I (non-obese) (n=24, BMI <25Kg/m2) and Group II (obese) (n=50, BMI P 25Kg/m2). Oxidative stress markers (MDA (malonaldehyde), NO2/NO3 (Nitrite/Nitrate) ratio, GSH (reduced glutathione) and GSH/GSSG (reduced glutathione/oxidized glutathione) ratio) were measured by high performance liquid chromatography (HPLC). Results: Obese women had signi{uFB01}cantly higher mean follicular {uFB02}uid MDA (P=0.006) as well as NO2/NO3 ratio (P = 0.004). BMI strongly correlated to follicular {uFB02}uid MDA (P=<0.01). MDA showed strong positive correlation to NO2/NO3 ratio (P=0.02). GSH and GSH/GSSG ratio showed a non- signi{uFB01}cant difference between the two groups (P=0.14 and 0.67, respectively). Clinical pregnancy rate was signi{uFB01}cantly higher in the non-obese group (87%) compared to the obese (43%) (P=<0.01, OR:13; 95% CI 3.54-52). With binary logistic regression, MDA was found to be a good predictor of the occurrence of pregnancy (P=<0.01). No signi{uFB01}cant differences were

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