Thesis (M.Sc.) - Cairo University - Faculty of Oral and Dental Medicine - Department of Oral and Maxillofacial Pathology

Objective: The aim of this study was to test the accuracy of toluidine blue (TB) in the assessment of intraoperative tumor margins after excision of oral squamous cell carcinoma (OSCC) in comparison to frozen sections (FS). Methods: a prospective study was conducted at Faculty of Dentistry and National Cancer Institute (NCI), Cairo University from July 2018 to June 2019 A total of thirty patient's surgical specimen of primary OSCC were included in the study. Total of 140 margins were analysis intraoperatively by TB and FS, the result was compared with final histopathological. Results: Of the 140 examined surgical margins there were 14 margins stained positive with TB. However, of these 14 margins only sex were true positives on final histopathology and 8 false positive, there were no false-negatives. TB staining had sensitivity and specificity of 100% and 94.03%, respectively. The diagnostic accuracy was 94.29% with a positive predictive value (PPV) of 42.86% and a negative predictive value (NPV) of 100%. While for FS there were three true positive, three false negative margins and no false positive. The FS had sensitivity and specificity of 50% and 100%, respectively. The diagnostic accuracy was 97.86%, with PPV of 100% and NPV of 97.81 %. Conclusion: The TB has high sensitivity to OSCC. It can be used as an adjunct to FS for the detection of positive margins of resected OSCC. The information that provided by TB can be used in guiding FS sampling from exact location instead of random FS biopsy sampling

Issued also as CD