Thesis (M.Sc.) - Cairo University - Faculty of Pharmacy - Department of Microbiology and Immunology

This study shows the possible synthesis of selenium nanoparticles (SeNPs) in aerobic optimized conditions using bacillus laterosporus (B. laterosporus) bacterial strain. B. laterosporus was used to reduce selenium ions (selenite anions) to SeNPs by fermentation in Luria - Bertani Enrichment (EM) medium. Optimization of fermentation conditions using two - level full factorial design was performed. SeNPs were further characterized by UV - Vis., DLS, TEM, FT - IR, EDX and XRD analysis. SeNPs synthesis by Gamma irradiated B. laterosporus cells at different radiation doses was reported. Evaluation the probability of B. laterosporus to synthesis SeNPs by fermentation in skimmed milk aerobically. A microtiterplate assay was used to evaluate the ability of SeNPs to inhibit the biofilm formation of Pseudomonas aeruginosa. Evaluating the antimicrobial activity of some antibiotic agents upon addition of SeNPs was performed

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