Thesis (M.Sc.) - Cairo University - Faculty of Veterinary Medicine - Department of Internal Medicine and Infectious Diseases

Diarrhea is a major problem of livestock in Egypt and throughout the world. Salmonella is one of the main etiological agents of infectious diarrhea and represents a zoonotic hazard worldwide. In the current study, a total number of 197 and 149 diarrheic sheep and goats of different age groups, at different seasons were clinically examined and from which rectal swabs were collected. Rectal swabs were bacteriologically examined for isolation of Salmonella. There were 5 Salmonella isolates recovered from diarrheic goats (3.36%) and 3 from diarrheic sheep (1.52%). The highest rate of Salmonella infection among the examined diarrheic sheep and goats was in Spring season and in (1-2.5 years) age group. Serological identification of the recovered Salmonella isolates revealed 6 different serotypes (Salmonella Bonariensis, Salmonella Kottbus, Salmonella Stanleyville, Salmonella Enteritidis, Salmonella Paratyphi A and Salmonella Ferruch) and 2 Untypable isolates. The recovered isolates were examined for their antimicrobial sensitivity and it was found that the highest resistance rates were recorded against ampicillin, amoxicillin, levofloxacin and ciprofloxacin with resistance rates 100%, 62.5%, 62.5% and 50%, respectively. While the highest sensitivity rates were detected for cefotaxime, gentamycin, amikacin, streptomycin, norfloxacin, sulphamethaxozle+trimethoprim, chloramphenicol and enrofloxacin with sensitivity rates 87.5%, 87.5%, 75%, 75%, 62.5%, 62.5% ,62.5% and 50%, respectively. PCR was applied for genotypic characterization of virulence genes (invA, Stn, bcfc and avrA). All isolates were positive for the 4 virulence genes. Also, PCR was used for confirmation of the existed resistance pattern in antibiogram test

Issued also as CD