Thesis (M.sc.)-Cairo nivsersity,2022.

Bibliography: p. 87-124.

responding author: 01112231511, Egypt, 11611, eman.50.fawzy@gmail.com Abstract Aims: to assess immunohistochemically the expression of CD38 in induced rat liver fibrosis after treatment with mesenchymal stem cells (MSCs) and validate their therapeutic potential. Study design: induction of fibrosis in liver of rats by using carbon tetrachloride (CCl4) then treatment with bone marraw MSCs (BM-MSCs). . Place and Duration of Study: National cancer institute, Cairo University. Methodology: Forty five adult rats were divided into 3 groups: control group A, CCl4-indused fibrosis group B and CCl4/BM-MSCs group C. After one month from transplantation, histological and immunohistochemistry examination of the liver tissue were performed. Results: Immunohistochemical staining for CD38 in liver showed negative cell membrane reactivity (0) in peri-portal and peri-venular areas in control group A. The CCl4-untreated rats (group B) without stem cell treatment showed moderate cell membrane reactivity (++) of hepatocytes peri-venular area. The CCl4-untreated rats with stem cell treatment (group c) showed negative cell membrane reactivity (0) for CD38 in peri-portal and peri-venular areas, CD38 expression semi-quantitatively analyzed by an experienced liver pathologist using a scoring system ranging from 0 to +3, where 0 indicates no expression of CD38 and +3 indicates the highest expression. Conclusion: The treated liver showed a degree of improvement of CCL4 induced hepatic injury, with decreased expression for CD38 in liver tissue when compared to the fibrotic livers. Keywords: CD 38, fibrosis, mesenchymal stem cell, animal model, CCl4 Abbreviations: mescenchymal stem cells (MSCs), cluster of differentiation (CD38), carbon tetrachloride ( CCL4)

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