Genetic polymorphism of some wool genes in Egyptian sheep breeds / Eman Hassan Ahmed Hassan ; Supervised Mahmoud Ahmed Amer , Haidan Mostafa Salem , Ibrahim Mohamed Farag

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Eman Hassan Ahmed Hassan

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TextLanguage: English Publication details: Cairo : Eman Hassan Ahmed Hassan , 2019Description: 63 P. : charts , photoghraphs ; 25cmOther title:

تعدد الأشكال المظهرية لبعض جينات الصوف في سلالات الأغنام المصرية [Added title page title]

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Dissertation note: Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology Summary: This study aimed to identify the genetic polymorphisms of some genes that have notable effect on wool quality traits in Egyptian sheep breeds, including staple length (STL), fiber diameter (FD), staple strength (STR) and clean fleece weight (CFW). KRT35 and KRT2.10 (KRT83) genes that code for the keratin intermediate-filament proteins (KRTs) along with microsatellite BFMS were targeted for this investigation. Data from PCR-SSCP assay of the KRT2.10 gene showed one PCR-SSCP pattern, confirmed by RFLP analysis using restriction enzyme (Bse3D I) showing one genotype of KRT2.10 gene with one undigested band at 191 bp. On the other hand, KRT35 using PCR-SSCP analysis revealed three different banding patterns corresponding to three genotypes AA, AB and AC (with frequencies 0.92, 0.02, 0.05 respectively). Sequencing analysis of different KRT35 genotypes revealed that A allele represents substitution of G/A at position 4016, allele B showed substitution of G/A at position 4056, and allele C revealed substitution of C/T at position 4027

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Holdings
Item type	Current library	Home library	Call number	Copy number	Status	Barcode
Thesis	قاعة الرسائل الجامعية - الدور الاول	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.12.21.M.Sc.2019.Em.G (Browse shelf(Opens below))		Not for loan	01010110078607000
CD - Rom	مخـــزن الرســائل الجـــامعية - البدروم	المكتبة المركزبة الجديدة - جامعة القاهرة	Cai01.12.21.M.Sc.2019.Em.G (Browse shelf(Opens below))	78607.CD	Not for loan	01020110078607000

Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Zoology

This study aimed to identify the genetic polymorphisms of some genes that have notable effect on wool quality traits in Egyptian sheep breeds, including staple length (STL), fiber diameter (FD), staple strength (STR) and clean fleece weight (CFW). KRT35 and KRT2.10 (KRT83) genes that code for the keratin intermediate-filament proteins (KRTs) along with microsatellite BFMS were targeted for this investigation. Data from PCR-SSCP assay of the KRT2.10 gene showed one PCR-SSCP pattern, confirmed by RFLP analysis using restriction enzyme (Bse3D I) showing one genotype of KRT2.10 gene with one undigested band at 191 bp. On the other hand, KRT35 using PCR-SSCP analysis revealed three different banding patterns corresponding to three genotypes AA, AB and AC (with frequencies 0.92, 0.02, 0.05 respectively). Sequencing analysis of different KRT35 genotypes revealed that A allele represents substitution of G/A at position 4016, allele B showed substitution of G/A at position 4056, and allele C revealed substitution of C/T at position 4027

Issued also as CD

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