Thesis (M.Sc.) - Cairo University - Faculty of Science - Department of Botany and Microbiology

The aim of this study was to evaluate the effect of some nutrient samples{u2013}chicken manure (CM), Nitrogen, phosphorus, potassium compound (NPK), Nitroben (Azotobacter/Azospirillum) (NT) and Azolla (AZ) as amendments for the bioremediation of oil polluted control soil (PCS) during 120 days. The organisms isolated from oil free soil (OFS) and oil samples were species of: Bacillus, Psuedomonas, Klebsiella, Escherechia, Staphylococcus, Micrococcus, Streptococcus, Burkholderia, Nitrosococcus, Aspergillus, Penicillium, Fusarium, Rhizopus and Mucor.The results of soil analysis showed that CM, NPK, NT and AZ considerably increased the phosphorus and pH of the soil to slightly alkaline condition which favors the biodegradation of the spent engine oil polluted soil. It was also concluded that nitrogen is a necessary nutrient for bacterial biodegradation activities.The highest percentage loss of spent oil was 79.07 by NPK followed by CM, NT and AZ with percentages 77.13, 73.37 and 70.43 respectively. These findings demonstrate the potential of chicken manure, Nitroben, Azolla as well as NPK to considerably increase the biodegradation of the spent lubricating oil polluted soil.The impact of the different nutrients (NPK, CM, NT and AZ) on the growth and performance of Glycine max L. (as a test plant), grown in soil polluted with the spent oil residue, were investigated after the experiment had run for 120 days

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