Thesis (M.Sc)-Cairo University, 2024.

Bibliography: pages 89-110.

The present study aimed to define the host range of the jasmine moth polyhedrovirus
(PaviNPV). Larvae of three insect species belong to the same family (Pyralidae), and larvae of other
two insects belonging to family (Noctuidae) were tested. The results showed that the recently
identified PaviNPV is specific to its homologous host (jasmine moth (JM), Palpita unionalis
(Hübner)). In order to determine the suitable larval instar and viral concentration needed for virus
production, the susceptibility of JM larval instars to PaviNPV was studied. The results showed that
the second, third, and fourth were highly susceptible to PaviNPV, and the mortality rate increased
with increasing virus concentration, however, fifth instar larvae was the least susceptible. The
results cleared that the JM fourth instar larvae and the concentration of 105 PIBs/mL of PaviNPV
were suitable combination for virus production. The addition of fluorescent brightener 28 (UNPA)
to PaviNPV increased the mortality rate, however, no significant increase could be detected in the
production rate. In order to increase the stability of PaviNPV under simulated sunlight (6 times the
same as natural sunlight), three natural additives, clove, henna, and rocket were mixed with
PaviNPV at a concentration of 107 PIB’s/mL and exposed to ultraviolet radiation for zero, 1, 3, and
5 hours under laboratory conditions. Clove and henna showed a potent UV protectant effect
compared to rocket. For comparison, the commercial biopesticide, Diple® 6.4% DF based on
Bacillus thuringiensis was used, which showed the highest mortality rate, followed by PaviNPV,
either plus clove or henna, for the tested four UV rays’ exposure periods. Field trials on olive crabs
were conducted to determine the persistence of PaviNPV in the presence or absence of clove in
comparison with Diple®. The results showed that Diple® and PaviNPV in the presence of clove
gave the highest reduction rate in larval population after 14 days of spraying. The study
recommends using PaviNPV in the integrated pest management (IPM) of the jasmine moth to
protect the olive crop.

هدفت الدراسة الحالية تحديد المدى العوائلي لفيروس البوليهيدروسز النووي لفراشة الياسمين PaviNPV كعامل مكافحة حيوي ضد يرقات فراشة الياسمين التي تصيب الزيتون. باختبار ثلاث أنواع من الحشرات التى تنتمي لنفس العائلة Pyralidae وكذلك يرقات حشرتين تنتميان لعائلة Noctuidae حيث أوضحت النتائج أن فيروس فراشة الياسمين (PaviNPV) الذي تم تعريفه حديثاً متخصص فقط على يرقات عائله الأصلي ( فراشة الياسمين) . تم تحديد الطور اليرقي المناسب والتركيز الفيروسي اللازم للإنتاج وهو العمر اليرقي الرابع لفراشة الياسمين و التركيز الفيروسي PaviNPV 105 بوليهيدرا / ملي. من أجل زيادة ثبات فيروس فراشة الياسمين تحت أشعة الشمس المقلدة, أظهرت النتائج أن إضافة المستخلص المائي للقرنفل والحنة ساعد الفيروس على البقاء فعالا لمدة أطول عن الفيروس بمفرده. تحت الظروف الحقلية على سرطانات حيث أظهرت النتائج أن مبيد الدايبل وفيروس PaviNPV مع القرنفل أعطى أعلى انخفاض في عدد اليرقات حتى 14 يوم من الرش على الزيتون.

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Text in English and abstract in Arabic & English.