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082			_a636.089
092			_a636.089 _221
097			_aPh.D
099			_aCai01.10.05.Ph.D.2022.Ta.C.
100			_aTahany Ahmed Ismail Alkolfat, _epreparation.
245			_aComparative evaluation of camel and sheep wharton jelly mscs therapy in induced chronic kidney disease model in dogs / _cby Tahany A.I. Alkolfat ; Supervised Prof.Dr.Hala Mohamed Farouk El Miniawy، Prof.Dr.Haithem Ali Mohamed Farghali, Prof.Dr.ina Sabry Abd El Fattah Essam Mohamed Ibraheem.
246			_aتقييم مقارن للعلاج بالخلايا الجذعية الميزينشيمية المستخلصة من الحبل السرى للجمال والأغنام فى نموذج مستحدث لمرض الكلى المزمن فى الكلاب
264		0	_c2022
300			_a109 P. : _billustrations _c25cm+ _eCd
336			_2rda content _atext
337			_2rdamedia _aUnmediated
338			_2rdacarrier _avolume
502			_a.Thesis (Ph.D)-Cairo Univsersity,2022.
504			_aBibliography: p. 96-109.
520			_achronic kidney disease (CKD) is a critical issue worldwide with an increasing incidence. Treatments available for CKD include dialysis and renal transplantation which have several drawbacks. Xenogeneic cell therapy provides a promising new option in the field of regenerative medicine by using cells from different tissues of animals as source for treating human diseases. Remarkable pre-clinical research needed for the demonstration of efficacy and safety of utilizing xenogeneic cells as therapeutic drug for the progress of this field. This study was carried out to investigate the possible therapeutic effect of both camel and sheep Wharton’s jelly MSC on canine CKD model. The study comprised two parts. Part I was a pilot study to determine the suitable method to induce surgically CKD model in dogs. Part II carried out to investigate the therapeutic effect of both CWJ-MSCs and SWJ-MSCs on the induced CKD model. Part I, the pilot study was applied in three dogs, each one represents one model. Model I, induced by excision of one pole of right kidney with removal of contralateral kidney after two weeks from first operation. Model II, induced by excision of two poles of right kidney with removal of contralateral kidney after two weeks from the first operation. Model III, was induced by ligation of two renal arterial branches of right kidney, with removal of contralateral kidney after two weeks from the first operation. The results of this study showed that model (2) was considered the best technique for developing a model of canine CKD with persistent uremia.. According to the IRIS staging system in dogs, model (2) represented stage three of CKD with moderate expression of kidney degenerative genes (KIM1& NGAL) and α-SMA in the interstitial tissue and moderate glomerulointerstitial nephritis. The dog in this model became uremic and remained stable for a long time which would allow experimental manipulation. Part II of the study, was performed on nine dogs divided into three groups. chronic kidney disease model induced in dogs by using 5/6 nephrectomy technique. The collected MSCs were isolated and cultured then identified by morphology and flow cytometric analysis. MSCs were injected intrarenal with ultrasonographic guidance in a dose of (5× 106 cells) in two doses. Serum was collected regularly for kidney function test. Kidney tissue specimens were collected for histopathology and identification of NGAL, KIM-1, and EGFR genes by real time PCR. Serum urea and creatinine levels were significantly decreased in the treated groups. The kidney function test recorded more decreased values in the group treated with CWJ-MSCs than that treated with SWJ-MSCs. On the level of histopathology, the lesion scores recorded improvement of glomerular lesions and fibrosis in all experimental cases in the group treated with CWJ-MSCs while in the group treated with SWJ-MSCs the renal lesions improvement varied between experimental cases especially medullary fibrosis. Remarkable thickening of glomerular basement membrane and focal mononuclear cellular reaction were obvious in this group. NGAL, KIM-1genes decreased and EGFR gene increased in all treated groups indicating regeneration. Conclusion: SWJ- MSCs transplantation improve renal function tests and enhance tubular regeneration and tissue repair with degree less than CWJ-MSCS but failed to decrease fibrosis. SWJ- MSCs transplantation in the induced CKD model incite interstitial mononuclear cells infiltration composed mainly of plasma cells and increase thickening of glomerular basement membrane, indicating immunological reaction occurrence.
520			_a • للخلايا الجذعيه الميزنشيمية المستخلصة من نسيج الحبل السري للجمل تأثير علاجي جيد على مرض الكلى المزمن المحدث جراحيا فى الكلاب من خلال تحسين وظائف الكلى وتحسن الصوره الباثولوجيه للمرض بالاضافه الى قدرتها على تقليل التليف. • للخلايا الجذعيه الميزينشيميه المعزوله من الاغنام القدره ايضا على تحسين وظائف الكلى وتحسين الصوره الباثولوجيه لمرض الكلى المزمن فى الكلاب ولكن تأثيرها اقل من نظيرتها المعزوله من الجمل بالاضافه الى عدم قدرتها على تقليل التليف. . • ربما تسببت الخلايا الجذعيه للاغنام فى رد فعل مناعى وذلك من خلال ارتشاح الخلايا الالتهابيه وحيده النواه على الاغلب خلايا البلازما فى النسيج البيبنى للكلى بالاضافه الى وجود سماكه فى الطبقه الجداريه لكبسوله بومان • حقن الخلايا مباشرا فى النسيج الكلوى تسبب فى وجود بعض الرضوض ربما حقن الخلايا قى الشريان الكلوى يكون له نتائج افضل. • واخيرا نحن فى حاجه الى مزيد من الدراسات لمعرفة المواد الالتهابية و انواعها و التى قد تصاحب العلاج بالخلايا الجذعية المنزنشيمة المستخرجة من الحبل السرى بالاضافه الى عمل دراسات مقارنه بين الخلايا الجذعيه الميزنشيميه المعزوله من الحبل السرى لكل من الجمل و الانسان وحيوان اخر وليكن الابقار . .
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650			_aCanine kidney disease (CKD)
653			_aSheep Wharton’s jelly mesemchymal stem cells (SWJ- MSCs)
700			_aHala Mohamed Farouk El Miniawy _ethesis advisor.
700			_aHaithem Ali Mohamed Farghali _ethesis advisor.
700			_aDrina Sabry Abd El Fattah Essam Mohamed Ibraheem _ethesis advisor.
900			_b01-01-2022 _cHala Mohamed Farouk El Miniawy _cHaithem Ali Mohamed Farghali _cDrina Sabry Abd El Fattah Essam Mohamed Ibraheem _UCairo University _FFaculty of veterinary medicine _DDepartment of Pathology
905			_aShimaa
942			_cTH _2ddc
999			_c163723