000			05706namaa22004451i 4500
003			OSt
005			20250223033312.0
008			241013s2023 |||a|||f m||| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposite
082	0	4	_a660.6
092			_a660.6 _221
097			_aPh.D
099			_aCai01.12.02.Ph.D.2023.Do.E
100	0		_aDoaa Mousa Mostafa Elhefnawei, _epreparation.
245	1	0	_aSelective biomarkers as predictive tools in investigating the efficacy of liposomal Coenzyme- Q10 in the autistic rat model / _cby Doaa Mousa Mostafa Elhefnawei ; Supervisors Prof. Dr. Mohamed Ali El-Desouky , Prof. Dr. Mohamed Ahmed Badawy , Prof. Dr. Ahlam Hosny Mahmoud , Prof. Dr. Mai Osman Mohamed.
246	1	5	_a/ Coenzyme-Q10 انتقاء المؤشرات الحيوية كدلالات تنبؤية لفاعلية ليبوزومات فى نموذج الجرذان التوحدى
264		0	_c2023.
300			_a121 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (Ph.D)-Cairo University, 2023.
504			_aBibliography: pages 93-121.
520			_aAutism spectrum disorder (ASD) is a neurodevelopmental condition with genetic and environmental origin marked by disturbance in social interaction and repetitive behaviors. Propionic acid (PPA) is considered as a successful autism animal model. Treatment of neurodegenerative diseases has tremendous therapeutic potential for Coenzyme-Q10 (CoQ10). Nevertheless, its low water solubility limited its therapeutic applications. Liposomal drug delivery possesses the benefits of biocompatibility, targeting organs and avoiding macrophages. The present study objective is assessing the effectiveness of CoQ10 and liposomal Coenzyme- Q10 (LCoQ10) on PPA-mediated autistic rat model. Liposomes are efficient for delivering CoQ10 across the brain-blood barrier (BBB) and reaching the target organ. PPA (500 mg/kg Bw in phosphate buffer saline) was given daily for five days to induce autism, then receiving therapy with CoQ10 (10 mg/kg BW) and LCoQ10 (2 mg/kg BW) for 30 days. The model was monitored for autism symptoms at various time intervals of (one, two and four weeks). Behavioral tests (Y- Maze and Open field), Gamma-amino butyric acid (GABA), Acetylcholine (ACh), Acetylcholinesterase (AChE) activity and antioxidant analysis were all accomplished in control, PPA and treated groups. Glial fibrillary acidic protein (GFAP), Calcium Voltage-Gated Channel Subunit Alpha 1 C (CACNA1C), Brain-Derived Neurotrophic Factor (BDNF), cAMP Response Element-Binding Protein (CREB) and Methyl-CpG-binding Protein 2 (MeCP2) gene expression were also performed. COMET assay and histological examination were also confirmatory. Superoxide dismutase (SOD), reduced glutathione (GSH), and total antioxidant capacity (TAC) revealed considerable increases via CoQ10 and LCoQ10 treatment in contrast to MDA. Moreover, they significantly down regulated GABA, AChE and the gene expression of GFAP, CACNA1C and BDNF. Furthermore, they up regulated ACh, the gene expression of CREB and MeCP2. They also improved DNA damage and PPA histopathological abnormalities and LCoQ10 showed even better results. In conclusion, targeting organs and extending retention time via LCoQ10 treatment may be a potential technique for limiting aberrant behaviors in PPA- induced autism rat model.
520			_aاضطراب طيف التوحد (ASD) هو واحد من أكثر الاضطرابات المربكة ل التي لم يتم تحديد سبب محدد فحسب ، بل لم يتم أيضا تحديد أي علاج نهائي لها تم العثور عليها حتى الآن. التوحد هو اضطراب يتم ملاحظته عادة على الطفل في سن مبكرة ، تؤثر على الجوانب التنموية المختلفة. تطورها غير طبيعي. إنه يظهر خللا في تفاعله الاجتماعي ، يتميز بتكرار بعض الأنماط السلوكية والتحديات في التواصل اللفظي وغير اللفظي مع الاخرين. على مدى العقود القليلة الماضية ، أظهر انتشاره ارتفاعا كبيرا. و الملاحظة التي شجعت العديد من الباحثين في جميع أنحاء العالم على محاولة استكشاف جميع جوانبه من المسببات إلى التشخيص والتدخل. ASD هو نوع من اضطراب النمو العصبي الذي يتميز بضعف التواصل الاجتماعي و التفاعلات المتكررة ، وكذلك المصالح المحدودة. يرتبط حمض البروبيونيك (PPA) بالسلوكية والعصبية المرضية و تشوهات كيميائية حيوية لوحظت في ASD.
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650		7	_aBiochemistry _2qrmak
653		0	_aAutism spectrum disorder _aliposomal Coenzyme Q10 _aGFAP _aCACNA1C _aBDNF _aCREB _aMeCP2
700	0		_aMohamed Ali El-Desouky _ethesis advisor.
700	0		_aMohamed Ahmed Badawy _ethesis advisor.
700	0		_aAhlam Hosny Mahmoud _ethesis advisor.
700	0		_aMai Osman Mohamed _ethesis advisor.
900			_b01-01-2023 _cMohamed Ali El-Desouky _cMohamed Ahmed Badawy _cAhlam Hosny Mahmoud _cMai Osman Mohamed _UCairo University _FFaculty of Science _DDepartment of Chemistry
905			_aShimaa _eHuda
942			_2ddc _cTH _e21 _n0
999			_c168380