000			06082namaa22004211i 4500
003			OSt
005			20250615113020.0
008			250504s2024 ua a|||frm||| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a621.366
092			_a621.366 _221
097			_aPh.D
099			_aCai01.24.03.Ph.D.2024.Na.P
100	0		_aNayera Mohamed Ali El Ghoubary, _epreparation.
245	1	0	_aPharmaceutical study of the photobiological and photothermal properties of sepia melanin nanoparticles / _cby Nayera Mohamed Ali El Ghoubary ; Supervisors Prof. Dr. Maha Fadel Mohamed, Prof. Doaa Ahmed Abdel Fadeel.
246	1	5	_aدراسة صيدلانية للخواص الضوئية البيولوجية و الحرارية للجزيئات النانوية لميلانين الحبار /
264		0	_c2023.
300			_a114 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (Ph.D)-Cairo University, 2024.
504			_aBibliography: pages 100-114.
520			_aMelanin is a natural photothermal biopolymer. It is considered the main chromophore for laser hair removal. The insolubility of melanin as an exogenous dye, in most solvents, limits its bioavailability and thus its clinical use. Melanin was extracted from a sepia ink sac and prepared as insoluble powdered sepia melanin (SM) which is identified by FTIR, 1H-NMR, thermogravimetric analysis (TGA), and scanning electron microscope (SEM). In our study, to overcome the solubility problems and increase the bioavailability of melanin for biomedical and cosmetic applications, SM was self-assembled using tween 80 into dispersed nanoparticles (SM-NP-Tw). Moreover, natural sepia melanin was loaded in different delivery systems (spanlastics and transfersomes) to be delivered to the hair follicles. The prepared SM-NP- Tw were fully characterized. The photothermal performance of SM-NP-Tw was assessed. Dark and photocytotoxicity of SM-NP-Tw was studied on HepG2 cells using two wavelengths (660 nm and 820 nm). The insoluble powdered (SM) exhibited a spherical nanoparticle-like shape as revealed by scanning electron microscope and was soluble only in an alkaline aqueous solution. TGA of SM showed high resistance to thermal degradation indicating good thermal stability. The prepared SM-NP-Tw exhibited a spherical shape with mean sizes of 308±86 nm and a zeta potential of −25 mv. The cell viability decreased significantly upon increasing the concentration and upon radiation at 820 nm. The results of UV–Vis spectroscopy and the photothermal performance revealed that melanin can absorb light in a wide range of wavelengths including near the IR region; thus, it can emit sufficient heat to kill cells through the photoheat conversion effects. Melanin spanlastics and transfersomes were prepared and characterized. Transmission electron microscopy (TEM) showed the spherical shape of the prepared vesicles with average particle sizes 252 and 262 nm and zeta potential of -22.5 and -35 mV for melanin spanlastics and melanin transfersomes, respectively. Histological examination of hair follicles and pilosebaceous glands for the irradiated and non- irradiated albino mice skin was studied after the application of the prepared formulations topically and subcutaneously. Qualitative statistical analysis was conducted and melanin transfersomes and melanin spanlastics showed significant damage to pilosebaceous glands and hair follicles with a p-value of 0.031 and 0.009 respectively. Sepia melanin nanoparticles self-assembled into tween-based nanostructures could be a promising natural platform for different applications including photothermal cancer therapy. As well, melanin nanoparticles loaded in transfersomes and spanlastics could be considered a promising approach for the laser removal of non-pigmented hair.
520			_aإن الهدف من هذه الرسالة هو تحضير الميلانين في أنواع مختلفة من موصلات الدواء. الميلانين هو بوليمر حيوي حراري ويعتبر الكروموفور الرئيسي لإزالة الشعر بالليزر. و لكن عدم قابلية ذوبان الميلانين كصبغة خارجية ، في معظم المذيبات ، يعد مشكلة كبيرة تحد من توافره البيولوجي وبالتالي استخدامه اكلينيكيا. تم استخراج الميلانين من الحبار وتحضيره كمسحوق غير قابل للذوبان (SM) و تمت دراسته. ولكى نتغلب على مشاكل الذوبان وزيادة التوافر البيولوجي للميلانين ، تم تحضيره في جسيمات نانوية ، وتم تحميل الميلانين في موصلات دواء مختلفة ليتم توصيل الميلانين إلى بصيلات الشعر.تم دراسة الخواص الصيدلية للميلانين و تقييم الأداء الحراري الضوئي لها. أيضا تم دراسة سمية الميلانين على الخلايا السرطانية فى المعمل في الظلام و فى وجود الضوء باستخدام جهاز ليزربطوليين موجيين (660 نانومتر و 820 نانومتر).
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650		7	_aLaser Sciences _2qrmak
653		0	_aMelanin _amelanin nanoparticles _acancer _alaser _ahair removal
700			_aMaha Fadel _ethesis advisor.
700			_aDoaa Ahmed Abdel Fadeel _ethesis advisor.
900			_b01-01-2024 _cMaha Fadel Mohamed _cDoaa Ahmed Abdel Fadeel _UCairo University _FNational Institute of Laser Enhanced Science _DDepartment of Laser Applications in Pharmaceutical Sciences
905			_aShimaa _eEman Ghareb
942			_2ddc _cTH _e21 _n0
999			_c171881