000			04563namaa22004451i 4500
003			OSt
005			20250702123547.0
008			250522s2024 ua a|||fr|m|| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a572
092			_a572 _221
097			_aM.Sc
099			_aCai01.12.02.M.Sc.2024.Ha.S.
100	0		_aHager Ahmed Sayed Ali, _epreparation.
245	1	0	_aSynthesis, Molecular Docking Studies And Biological Evaluation Of Some Unsaturated Carbonyl Derivatives / _cBy Hager Ahmed Sayed; Supervisor Prof. Dr. Ismail Abdelshafy Abdelhamid, Dr .Nada Sabry Ibraheem Abd Elhady, Dr.Marwa Mohamed Mohamed Ahmed Sharaky.
246	1	5	_aالتحضير ودراسات الالتحام الجزيئي والتقييم البيولوجي لبعض مشتقات الكربونيل غير المشبعة الجديدة /
264		0	_c2024.
300			_a80 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (M.Sc.) -Cairo University, 2024.
504			_aBibliography: pages 71-80.
520			_aEight Novel chalcones were synthesized and their structures were confirmed by different spectral tools. All the prepared compounds were subjected to SRB cytotoxic screening against several cancer cell lines. Compound 5c exerted the most promising effect against MCF7 and HEP2 cells with IC50 values of 9.5 and 12 µg/mL, respectively. Real-time PCR demonstrated the inhibitory effect of compound 5c on the expression level of Antigen kiel 67 (KI-67), Survivin, Interleukin-1beta (IL-1B), Interleukin-6 (IL-6), Cyclooxygenase-2 (COX-2) and Protein kinase B (AKT1) genes. Flow-cytometric analysis of the cell cycle indicated that compound 5c stopped the cell cycle at the G0/G1 phase in MCF7 treated cells. ELISA assay showed that Caspase 8, Caspase 9, P53, BAX, and Glutathione (GSH) were extremely activated and Matrix metalloproteinase 2 (MMP2), Matrix metalloproteinase 9 (MMP9), BCL2, Malondialdehyde (MDA), and IL-6 were deactivated in 5c treated MCF7 and HEP2 cells. Wound healing revealed that chalcone 5c reduced the ability to close the scrape wound and decreased the number of migrating MCF7 and HEP2 cells compared to the untreated cells after 48 h. Theoretical molecular modeling against P53 cancer mutant Y220C and Bcl2 showed binding energies of -22.8 and -24.2 Kcal/mole, respectively which confirmed our ELISA results.
520			_aلقد تم تحضير ثمانية من مركبات الشالكون وتم التاكد منهم باستخدام الوسائل الطيفية المختلفه,لقد تم عمل الدراسات النظريه مثل التصميم الجزيئي لتحديد ميكانزم عمل هذه المركبات ,نتائج التاثير السمي علي الخلايا اوضحت ان المركب 5c له تاثير قوي علي الخلايا MCF7و HEP2 وقد تم اختيارهم لعمل الدراسات الجزيئيه ,لقد اوضح تحلبل البي سي ار الكمي ان المركب تسبب في نقص التعبير الجيني لجينات ki67 and survivin، IL-1B، IL-6، COX-2، AKT1 ,التحليل الفلوسيتموتري اوضح ان توقف دورة الخليه لخلايا سرطان الثدي قد تم عند مرحلة G0/G1 , وقد اوضحت نتائج تحليل الاليزا زيادة نشاطCaspase 8،Caspase 9 ، P53، BAX و GSH,وتقليل نشاط MMP2,MMP9,BCL2,MDA and IL-6 واظهر فحص التئام الجروح ان مركب 5C قلل من عدد خلايا الثدي والحنجره السرطانيه المهاجره الي مكان الجرح.
530			_aIssued also as CD
546			_aText in English and abstract in Arabic & English.
650		7	_aBiochemistry _2qrmak
653		0	_aChalcones _aAntioxidant _aAnti-inflammatory
700	0		_aIsmail Abdelshafy Abdelhamid _ethesis advisor.
700	0		_aNada Sabry Ibraheem Abd Elhady _ethesis advisor.
700	0		_aMarwa Mohamed Mohamed Ahmed Sharaky _ethesis advisor.
700	0		_aTayser Abdelkhalek _ethesis advisor.
900			_b01-01-2024 _cIsmail Abdelshafy Abdelhamid _cNada Sabry Ibraheem Abd Elhady _cMarwa Mohamed Mohamed Ahmed Sharaky _cTayser Abdelkhalek _UCairo University _FFaculty of Science _DDepartment of Chemistry
905			_aEman El gebaly _eEman Ghareb
942			_2ddc _cTH _e21 _n0
999			_c172302