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005			20251103111857.0
008			251021s2024 ua a|||frm||| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a660.62
092			_a660.62 _221
097			_aPh.D
099			_aCai01.12.05.Ph.D.2024.Sa.M
100	0		_aSafia Ahmed Amin, _epreparation.
245	1	0	_aMetallic nanoparticles production by Actinomycetes and Their Impact against Some human Pathogen / _cby Safia Ahmed Amin ; Supervisors Prof. Dr. Mohamed E. A. Dawoud, Dr. Mohamed Mahmoud Fathy, Dr. Khalid Ibrahim Abd-Elghany, Dr.Dina El-Basyoni.
246	1	5	_aإنتاج الجزيئات النانونية من الأكتينومايسيتات ودراسة تأثيرها ضد بعض الميكروبات الممرضة للإنسان
264		0	_c2024.
300			_a83 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (Ph.D)-Cairo University, 2024.
504			_aBibliography: pages 55-83.
520		3	_aBackground: The Study Investigated Metallic Nanoparticles Synthesized extracellularly. Using actinomycetes isolated from different environments as (The Mediterranean Sea, Some Fields, stagnant water, underground water, and rainfall water). Screening For actinomycetes For biosynthesis of metallic nanoparticles revealed that among collected thirty-five sample only six actinomycetes could obtained and among six Tested actinomycetes, only three exhibited The ability to synthesis AgNPs with antibacterial activity. These potent isolates were identified as Streptomyces albus strain smmdk14 (OR685674), Micromonospora maris strain smmdk13 (OR685672), and Arthrobacter crystallopoietes strain smmdk12 (OR685674), based on their characteristics.. On the basic of morphological and Physiological Properties Together with 16rRNA sequences.The biosynthesized AgNPS significantly affected and inhibited The growth of medically important Pathogenic bacteria (E-coli spp , Pseudomonas spp , Klebsiella spp ,Enterococcus spp ,Staphylococcus aureus, Acintobacter spp). biosynthesized AgNPS. were achieved by addition of silver nitrate (1 mM) To Culture Supernatant and monitored by Color Change after Incubation Then Characterization by Ultra visible Spectrophotometer, Fourier Transform Infrared (FTIR)Spectroscopy and transmission election microscopy (TEM). The obtained AgNPS Spherical and particles Size (6.46nm-24.7nm).optimization of Culture employed for production of AgNPs. Antimicrobial effect of AgNPs comparison with different antibiotics ,cytotoxicity and wound healing activities were evaluated. The microbiologically synthesized AgNPs act as antibacterial agent against six clinical pathogenic isolates. Moreover, silver nanoparticles exhibited a significant antibacterial effect more than many used antibiotics in study. Wound healing results illustrated that fibroblasts advanced toward the opening to close the scratch wound by roughly 66.4% after a 24-h exposure to Ag-NPs, greatly accelerating the wound healing process. In conclusion, a silver nanoparticles was successfully prepared and exhibited antibacterial, and wound healing activities that could be used in the medical field.
520		3	_aتعرف شعبه الاكتينوميسيتات بانتشارها الواسع في جميع الاوساط المحيطه حيث يمكن عزلها من التربه الطينيه والصحراويه والمياه المالحه والعذبه وقد تزايد في الاونه الاخيره المقاومه البكتريه للمضادات الحيويه وقداصبح ذالك من اكبر التحديات البيولوجيه ولذالك اصبح اللجوء للبدائل والبحث عن مركبات عضويه امنه ومستخرجه من الكائنات الدقيقه وتكون اكثر مقاومه للبكتريا الممرضه وقد اتجه العلماء في العديد من الابحاث الي عزل وتعريف الاكتينومايستات واستخراج العديد من المركبات الحيويه النافعه والتي لها قدره عاليه في مقاومه العديد من البكتريا الممرضه للانسان.
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650		0	_aMicrobiology
650		0	_aالميكروبيولوجيا
653		1	_aActinomycetes _ananoparticles _aCharacterization _aantibacterial activity _aWound healing
700	0		_aMohamed E. A. Dawoud Dr. Dr. Dr. _ethesis advisor.
700	0		_aMohamed Mahmoud Fathy _ethesis advisor.
700	0		_aKhalid Ibrahim Abd-Elghany _ethesis advisor.
700	0		_aDina El-Basyoni _ethesis advisor.
900			_b01-01-2024 _cMohamed E. A. Dawoud _cMohamed Mahmoud Fathy _cKhalid Ibrahim Abd-Elghany _cDina El-Basyoni _UCairo University _FFaculty of Science _DDepartment of Botany and Microbiology
905			_aShimaa _eEman Ghareb
942			_2ddc _cTH _e21 _n0
999			_c175129