000			06916namaa22004451i 4500
003			EG-GICUC
005			20251026135809.0
008			251026s2025 ua a|||frm||| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a616.01
092			_a616.01 _221
097			_aM.Sc
099			_aCai01.08.06.M.Sc.2025.Mo.M
100	0		_aMohamed Taha Abdelaziz, _epreparation.
245	1	0	_aMolecular and computational investigation of the skin microbiota associated with Pityriasis infection / _cby Mohamed Taha Abdelaziz ; Supervision Dr. Ramy Karam Aziz, Dr. Mariam Hassan Haikal, Dr. Dalia Ali Eldamacy.
246	1	5	_aدراسات جزيئية وحاسوبية للتجمعات الميكروبية الجلدية المرتبطة بعدوي النخالية المبرقشة
264		0	_c2025.
300			_a72 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (M.Sc)-Cairo University, 2025.
504			_aBibliography: pages 61-72.
520		3	_aPityriasis versicolor is a superficial fungal infection that causes skin pigmentation lesions, mostly without any severe symptoms but with a high prevalence and incidence of recurrence. An altered skin microbial community with an increase in Malassezia species, predominately Malassezia furfur, together with the transformation of those pityrosporum Malassezia to their mycelial form, are reported as the main cause of the disease. Despite the substantial advances in studying the human skin microbiome over the past two decades, the distribution of M. furfur in different skin microbiomes, and the involvement of the skin bacteriome in pityriasis versicolor are not conclusive. This study was initiated to systematically determine the distribution of M. furfur in sequenced skin metagenomes and to experimentally investigate its association with skin bacteriome profiles, which may provide clues to the role of skin microbiota balance in the treatment of pityriasis versicolor. First, publicly available skin metagenomes were computationally analyzed to systematically determine the distribution of M. furfur and its relative abundance at different skin sites. Using BLASTN to map newly proposed marker genes to representative metagenomic datasets allowed the estimation of M. furfur relative abundance, which indicated a relative enrichment of M. furfur in retro auricular crease, antecubital fossa, and forehead samples. Among skin categories, sebaceous areas were the most significantly enriched in M. furfur, while in terms of exposure/occlusion, exposed areas had the highest relative abundance. Second, an experimental case-control study investigated how the skin bacteriome, its dysbiosis, and fungal colonization might be related to pityriasis versicolor infection. The study was conducted on 20 skin swabs (taken from the neck to the subclavius area) from ten patients with pityriasis versicolor and six swabs from healthy volunteers (healthy controls). For each patient, one swab was taken from the lesion, while the other from a non-lesion area. The bacteriome of the 26 samples was profiled by 16S rRNA amplicon sequencing. Bioinformatics analysis indicated that samples from healthy control were more diverse and significantly differed (p-value < 0.05) in their bacterial composition from patients with pityriasis versicolor samples (lesion and non-lesion samples). Betaproteobacteria and Alphaproteobacteria had lower relative abundance in pityriasis versicolor patient skin, while Gammaproteobacteria was slightly more abundant. On the genus level, Ochrobactrum was relative more abundant in healthy skin samples, while Facklamia was relatively more abundant in lesion and non-lesion samples of patients with pityriasis versicolor. In conclusion, skin fungal alteration associated with pityriasis versicolor correlated with remarkable skin bacterial dysbiosis, not only in skin lesions, but also in the non-lesion areas. This finding suggests that bacterial dysbiosis may correlate with pityriasis versicolor infection and recurrence. Moreover, identifying commonly altered disease-associated bacterial taxa is a first step towards potential intervention to restore normal diversity, thus reducing the risk pityriasis versicolor and improving patient recovery.
520		3	_a تحتوي الرسالة على اربعة فصول. الفصل الاول يتضمن مقدمة حول الطرق المختلفة لتشييد مشتقات الثيينو]٢,٣-د[بيريميدين، ومقدمة موجزة عن موت الخلايا المبرمج، وإنزيمات مستقبلات التيروزين كيناز، وموت الخلايا المبرمج، بالإضافة إلى النشاط المضاد للسرطان لمشتقات الثينوبيريميدين. الفصل الثاني يعرض أهداف البحث وعرض المخططات التي توضح الطرق العملية التي صممت للحصول على هذه المركبات. الفصل الثالث يتضمن المناقشة النظرية للجزء العملى، ومناقشة الأنشطة المضادة للسرطان والأنشطة الإنزيمية للمركبات الجديدة، ودراسة النمذجة الجزيئية، ومناقشة موجزة عن علاقة تركيب المركبات بفاعليتها. الفصل الرابع يشمل الخطوات العملية التي تم تنفيذها لتحضير المركبات الوسيطة والمستهدفة بالتفصيل، بالإضافة إلى خصائص المركبات التي تم تشييدها، وتحليل العناصر والبيانات الطيفية لهذه المركبات. هذا الفصل يغطي أيضا النشاط المضاد للسرطان في المختبر وفاعلية المركبات التي تم تشييدها حديثًا ضد إنزيم FLT-3 ، ونشاط موت الخلايا المبرمج للمركبات الأكثر فاعلية ودراسة النمذجة الجزيئية لها.
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650		0	_aMicrobiology
650		0	_aالميكروبيولوجيا الطبية
653		1	_a16S rRNA gene sequencing _aBLASTN _aDysbiosis _aMalassezia furfur _aMetagenomics _aPityriasis versicolor
700	0		_aRamy Karam Aziz _ethesis advisor.
700	0		_aMariam Hassan Haikal _ethesis advisor.
700	0		_aDalia Ali Eldamacy _ethesis advisor.
900			_b01-01-2025 _cRamy Karam Aziz _cMariam Hassan Haikal _cDalia Ali Eldamacy _UCairo University _FFaculty of Pharmacy _DDepartment of Microbiology and Immunology
905			_aShimaa
942			_2ddc _cTH _e21 _n0
999			_c175210