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049			_aDeposit
082	0	4	_a632
092			_a632 _221
097			_aM.Sc
099			_aCai01.07.16.Ph.D.2025.Im.R
100	0		_aIman Amer Abdelhafiz Amer, _epreparation.
245	1	0	_aRole of some rhizobacteria in controlling agrobacterium tumefaciens the causal agent of crown gall disease / _cby Iman Amer Abdelhafiz Amer ; Supervisors Dr. Maurice Sabry Mikhail, Dr. Maryan Makram Yousef, Dr. Ashraf Fathy Abd El-Rahman.
246	1	5	_aدور بعض الريزوبكتيريا في مكافحة tumefaciens Agrobacterium ال مسبب لمرض التدرن التاجي
264		0	_c2025.
300			_a138 + 65 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (Ph.D)-Cairo University, 2025.
504			_aBibliography: pages 107-139.
520		3	_aCrown gall caused by Agrobacterium tumefaciens (synonym Rhizobium radiobacter) is one of the most dangerous bacterial diseases affecting the production of fruit tree nurseries in Egypt and many countries of the world. A study of 55 bacterial isolates from the rhizosphere of stone fruit plants showed that ten isolates of 1-aminocyclopropane-1- carboxylic acid (ACC) deaminase-producing rhizobacteria were identified as Pseudomonas strains. Co-inoculating castor bean (Ricinus communis L.) and kalanchoe (Kalanchoe sp.) plants with A. tumefaciens and ACC-producing isolates of Pseudomonas vancouverensis reduced tumor formation in both plants. Also, the secondary metabolites of six of the 55 isolates inhibited the growth of A. tumefaciens, as indicated by the inhibition zone test. The results showed that those six isolates reduced the diameter and weight of tumors produced on kalanchoe and tomato (Solanum lycopersicum L.) plants. The six isolates were identified as Klebsiella, Pantoea (four isolates), and Enterobacter. GC-MS analysis of ethyl acetate (EtOAc) extracts of the metabolites of four selected isolates of the six isolates found that 4H-pyran-4-one, 2,3-dihydro-3,5-dihydroxy-6-methyl- is the major component in the metabolites of Klebsiella and Enterobacter and bis (2-ethylhexyl) phthalate in Pantoea metabolites. Meanwhile, LC-ESI-TOF-MS analysis found that hydroquinone and 4-methylcatechol are two of the major components in the EtOAc extracts of the metabolites of all four selected isolates. Meanwhile, two isolates (BPeK8 and BpeL6) that produce VOCs were selected from 55 isolates to evaluate their ability to inhibit the growth of A. tumefaciens and decrease crown gall disease. VOCs of BPeK8 and BPeL6 strongly affected the growth of A. tumefaciens. Scanning electron microscopic (SEM) analysis showed that the VOCs of the two isolates caused morphological abnormalities in A. tumefaciens cells. The results showed that VOCs produced by BPeK8 and BPeL6 can significantly decrease the pathogenicity of A. tumefaciens cells. The two isolates BPeK8 and BPeL6 were identified as Bacillus subtilis and Pseudomonas aeruginosa, respectively. GC-MS analysis identified the major peaks of VOCs produced by B. subtilis BPeK8 as dimethylamine, hydrazinecarboxamide, and acetoin. The major peaks of VOCs produced by P. aeruginosa BPeL6 were identified as oxalic acid, 1-butanol, 2-methyl-, (S)-, and cinnamaldehyde, (E)-. These results showed that many rhizobacteria species can control crown gall caused by A. tumefaciens by different mechanisms.
520		3	_aيعتبر مرض التدرن التاجي المتسبب عن بكتيريا tumefaciens Agrobacterium من أخطر الأمراض البكتيرية التي تؤثر على إنتاج مشاتل الأشجار ال م ثمرة في مصر والعديد من دول العالم .أظهرت دراسة ل 55 عزلة بكتيرية من منطقة الريزوسفير لنباتات الفاكهة ذات النواة الحجرية أن عشر عزلات منتجة ل إنزيم 1 أمينوسايكلوبروبان 1 كربوكسيلك أسيد . (ACC)تم التعرف عليها كعزلات من spp. Pseudomonas . أدى التلقيح المختلط لنبات الخروع ونبات الكالانشو إلى تقليل تكوين الأورام في كلا النباتين . كما وجد أن المستقلبات الثانوية ل 6 من العزلات قد عملت على تثبيط نمو tumefaciens A. وعزلتين منتجتان للمركبات العضوية المتطايرة
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650		0	_aPlant Pathology
650		0	_aأمراض النبات
653		0	_aBiocontrol _acrown gall _aRhizobium radiobacter _aPseudomonas spp _aKlebsiella _aPantoea _aEnterobacter _aBacillus subtilis _aPseudomonas aeruginosa _aacdS gene
700	0		_aMaurice Sabry Mikhail _ethesis advisor.
700	0		_a Maryan Makram Yousef _ethesis advisor.
700	0		_aAshraf Fathy Abd El-Rahman _ethesis advisor.
900			_b01-01-2025 _cMaurice Sabry Mikhail _cMaryan Makram Yousef _cAshraf Fathy Abd El-Rahman _UCairo University _FFaculty of Agriculture _DDepartment of Plant Pathology
905			_aShimaa
942			_2ddc _cTH _e21 _n0
999			_c175432