000			05696namaa22004571i 4500
003			EG-GICUC
005			20260124130811.0
008			260122s2025 ua a|||frm||| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a632.05
092			_a632.05 _221
097			_aPh.D
099			_aCai01.07.16.Ph.D.2025.Ta.N
100	0		_aTarek Gomaa Abdel-Gaied Abdou, _epreparation.
245	1	0	_aNew bacterial blight of strawberry in Egypt : _bthe causal organism and its control / _cby Tarek Gomaa Abdel-Gaied Abdou ; Supervisors Dr. Maurice Sabry Mikhail, Dr. Maryan Makram Youssef, Dr. Hamdy Ibrahim Seif El-Nasr, Dr. Hassan Abd-EL-Khair Hassan.
246	1	5	_aمرض لفحة الفراولة الجديد في مصر : _bالميكروب المسبب ومكافحته
264		0	_c2025.
300			_a181 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (Ph.D)-Cairo University, 2025.
504			_aBibliography: pages 146-181.
520		3	_aThis study aimed to isolate and identify the microbes associated with the strawberry leaf blight disease symptoms. Samples of infected strawberry leaves were collected from three governorates: Ismailia, Beheira and Kalubia. The isolation process was carried out from naturally infected samples according to standard bacteriological methods. Twelve bacterial isolates were obtained as follows: four isolates from each governorate. The isolate IPa2 from Ismailia and the isolate BPa6 from Beheira were the most aggressive isolates causing the disease symptoms on healthy strawberry leaves. Therefore, according to the cultural, physiological, biochemical characteristics the isolates IPa2 and BPa6 were selected to identify using 16S rRNA sequencing the two selected isolates were identified as Pantoea ananatis with similatriy level of 97.05% with accession number (MH_127816.1) for IPa2, as well as was the isolate BPa6 with similatriy of 97.05% with accession number (NR_026045.1). The 16S rDNA sequencing were depositied in the Gen bank nucleotide data bases under accession number (MH_127816.1) &(NR_026045.1), respectively. In vitro experiments, three bacterial culture filtrates of B. subtilis and Ps. fluorescens ; three essential oils; seven plant extracts and some safe chemicals were suppressive to the growth of P. ananatis. The highest inhibition zones of 1.637, 2.158, 1.169,1.694cm were resulted form Ps. fluorescens, thyme, garlic extract and salicalic acid, respectively. In field experiments, all the applied treatments, either as root dipping or foliar spraying, significantly reduced the strawberry leaf blight severity. The bacterial culture filtrates of B. subtilis was the most effective treatment that decreased the disease severity to 19.0 and 14.0%. In general, foliar spray application of the tested treatments were more effectval than root dipping application. The application of all the tested treatments, enhanced the estimated plant growth and yield parameters as well as the activities of peroxidase, polyphenole oxidase and β-1,3 glucanase enzymes and fruits quality parameters compared to the infected control.
520		3	_aهدفت هذه الدراسة إلى عزل وتعريف مسبب مرض لفحة أوراق الفراولة. جمعت العينات المصابة من ثلاث محافظات الإسماعيلية والبحيرة والقليوبية و إجراء عملية العزل وفقاً للطرق البكتريولوجية القياسية. حصلنا على اثنتي عشرة عزلة بكتيرية: أربعه من الإسماعيلية، أربعه من البحيرة، أربعه من القليوبية. كانت العزلتين Pa2 و Pa6 الاقوي من حيث شدة حدوث الأعراض على أوراق الفراولة السليمة. لذلك تم اختيارهما للتعريف باستخدام 16 SrRNA على أنهم P. ananatis بنسبة تشابه 97.05٪ برقم دولي (MH_127816.1) للعزلة Pa2 و العزلة Pa6 بنسبة تشابه 97.05٪ برقم دولي (NR_026045.1). بالمعمل، كان راشح مزارع بكتيريا B. subtilis و Ps. fluorescens اعلي تثبيطا ضد بكتيرياananatis .P لكن المستخلصات النباتية كانت الاقل. بالحقل أدت المعاملات لتقليل الاصابه بالمرض وحسنت النمو الخضري والمحصولي وجودة الثمار و زيادة النشاط الإنزيمي بالنباتات
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650		0	_aPlant diseases
650		0	_aأمراض النبات
653		1	_aPantoea ananatis _aLeaf blight _aStrawberry _aEgypt _aDNA sequencing _aControl _abio-control agents _aEssential oils _aPlant extracts _aField application and fruits quality _aبانتويا اناناتس _aمرض لفحة الأوراق
700	0		_aMaurice Sabry Mikhail _ethesis advisor.
700	0		_aMaryan Makram Youssef _ethesis advisor.
700	0		_aHamdy Ibrahim Seif El-Nasr _ethesis advisor.
700	0		_aHassan Abd-EL-Khair Hassan _ethesis advisor.
900			_b01-01-2025 _cMaurice Sabry Mikhail _cMaryan Makram Youssef _cHamdy Ibrahim Seif El-Nasr _cHassan Abd-EL-Khair Hassan _UCairo University _FFaculty of Agriculture _DDepartment of Plant Pathology
905			_aShimaa
942			_2ddc _cTH _e21 _n0
999			_c177970