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003			EG-GICUC
005			20260303092348.0
008			260218s2025 ua a|||frm||| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a630.24
092			_a630.24 _221
097			_aPh.D
099			_aCai01.07.04.Ph.D.2025.Sa.I
100	0		_aSamar Ahmed Abdelaziz Ahmed, _epreparation.
245	1	4	_aThe induction and selection of abiotic stresses tolerance in balady apple through tissue culture technique / _cby Samar Ahmed Abdelaziz Ahmed ; Supervisors Dr. Emam Abdel Mobdi Abdel-Rahim, Dr. Mohamed Magdy Rashed, Dr. Rania Ahmed Mahmoud.
246	1	5	_aالإستحداث والإنتخاب لصفة تحمل الإجهادات البيئيه فى أصل التفاح البلدى باستخدام تقنية زراعة الأنسجة
264		0	_c2025.
300			_a156 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (Ph.D)-Cairo University, 2025.
504			_aBibliography: pages 139-156.
520		3	_aThe apple (Malus domestica) is a fruit that is cultivated extensively and has significant economic value worldwide. Soil salinization and drought consider to be major threats to apple production in some regions. The study aimed to facilitate the detection and selection of salt and drought stresses tolerance in apple (Malus domestica) seedling clones through tissue culture technique. Open pollinated mature seeds of local Egyptian ‘Balady’ apple were collected from ripped fruits of one tree and used as establishment plant materials. Every surface-sterilized seed was cultivated independently and assigned a distinct serial number. None of the micro-propagated individuals was typically as compared to each other or with the mother-plant according to similarity indices based on Inter Simple Sequence Repeats (ISSRs), where the most dissimilar genotype to the mother-plant was MS5 represents 84.9% similarity. After three consecutive subcultures, salt stress was achieved by adding different concentrations of NaCl (0, 1000, 2000 and 3000 ppm) to the proliferation medium, while stimulated drought stress by adding different concentrations of mannitol (0, 50, 100, 150 and 200 mM). The MS5 clone maintained relatively higher number of shoots, average shoot height and fresh weight together up to 3000 ppm NaCl comparing to other tested clones which indicated the superiority of MS5 clone under severe salt stress conditions. A specific positive marker of 290 bp by HB12 ISSR exclusively detected in the most salt-tolerant MS5 clone may support early selection for salinity tolerance. The most salt-tolerant MS5 clone was characterized by the least average of reducing sugars and total phenols accompanied by the minimum overall antioxidant activity, with stimulated activities of superoxide dismutase (SOD), peroxidase (Px) and catalase (CAT) antioxidative enzymes over control as affected by elevated salt concentration up to 3000 ppm NaCl. Data of three common differentially expressed genes (DEGs) revealed that, protein phosphatases PP2C-77a and ABA insensitive transcription factor ABI5-5b were downregulated while serine/threonine-protein kinase SAPK3 was overexpressed in MS5 genotype under severe salt stress conditions. The MS18 clone was the most adversely affected by osmotic stress induced by mannitol additives indicating its sensitivity to drought stress compared to other clones which responded to drought at varying levels. Also, it was the most susceptible clone under saline conditions, which indicates potential overlapping tolerance pathways of both dehydration and salinity abiotic stresses. The study confirmed the viability of in vitro screening for salt- or drought-tolerant genotypes within apple seedling populations.
520		3	_aباستخدام تقنية زراعة الأنسجة جمعت بذور ناضجة ملقحة تلقيحا حرا لتفاح "بلدي" المصري المحلي (Malus domestica) من ثمار شجرة واحدة واستخدمت كمواد نباتية تأسيسية. زُرعت البذوروتم تعيين رقم تسلسلي مميز لها. ولم تتماثل الأفراد المتكاثرة بدقة مع بعضها أو مع النبات الأم وفقًا لمؤشرات التشابه القائمة على التكرارات التسلسلية البسيطة (ISSRs)، و ثبت جدوى الفحص المختبري لتحمل الجفاف، ووجود نمط جيني عالي التحمل للملوحة بين شتلات التفاح. و امكان استخدام الفحص المختبري للجينات عالية التحمل لصفتي الملوحة و الجفاف بين مجموعات شتلات التفاح لانتاج شتلات تتحمل ظاهرتي الاجهاد البيئي الملوحة و الجفاف.
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650		0	_aAgricultural Biochemistry
650		0	_aالكيمياء الحيويه
653		1	_aApple _asalinity _adrought _ain vitro _aseedling clones _aantioxidant _aISSR _aالتفاح _aالملوحة
700	0		_aEmam Abdel Mobdi Abdel-Rahim _ethesis advisor.
700	0		_aMohamed Magdy Rashed _ethesis advisor.
700	0		_aRania Ahmed Mahmoud _ethesis advisor.
900			_b01-01-2025 _cEmam Abdel Mobdi Abdel-Rahim _cMohamed Magdy Rashed _cRania Ahmed Mahmoud _dMohamed Shaaban Hassan _dMagdy Abdel Aleem Mohamed Shallan _UCairo University _FFaculty of Agriculture _DDepartment of Biochemistry
905			_aShimaa _eEman Ghareb
942			_2ddc _n0 _cTH _e21
999			_c178593