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040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a632.95
092			_a632.95 _221
097			_aM.Sc
099			_aCai01.07.04.M.Sc.2025.Fa.B
100	0		_aFatma El-Zahraa Samir Sobhy, _epreparation.
245	1	0	_aBiochemical studies of caper bush capparis spinosa l. by in vitro culture / _cby Fatma El-Zahraa Samir Sobhy ; Supervisors Dr. Sherif Helmy Ahmedm, Dr. Rania Saber Yousef, Dr.Shreif Said Saleh Ahmed.
246	1	5	_aدراسات كيميائية حيوية علي نبات الكباريس باستخدام زراعة الانسجة
264		0	_c2025.
300			_a103 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_a Thesis (M.Sc)-Cairo University, 2025.
504			_aBibliography: pages 87-103.
520		3	_a Capparis spinosa L., an edible medicinal plant, is rich in flavonoids. Production of secondary metabolites preferentially uses amino acids as precursors using in vitro culture techniques. This study aimed to evaluate the effect of amino acids as elicitors on morphological characterization,non-antioxidant compounds accumilation, antioxidant enzymes, and anticancer activity Capparis spinosa was in vitro cultured on MS media containing three amino acids included; tryptophan, glutamine or phenylalanine at 100, 200, and 300 mg/L and compared with free amino acid culture (as control). After four weeks morphological characteristics were determined, some phytochemicals panel and rutin were evaluated by spectrophotometric and HPLC, respectively. The hepatocellular carcinoma cell line HepG2 was utilized to validate the anti cancer activity and in silico studies. The results showed that using colorex 15% resulted in 91.67% of disinfection.Applying 6-Benzylaminopurine(BAP) at 0.8 mg/L in MS media gave the highest shootlet length(4.03cm) ,but kinetin at 04 mg/L gave the highest shootlet number (6.11shoot/explant) . the most significant result (100%) to callus induction was achieved with MS medium supplemented with 0.5 mg/L 2,4-D for leaves.The MS media supplemented with 200 mg/L of tryptophan produced the most significant results, with an average shootlet number of 34.67. The explants grown on MS media supplemented with 100 mg/L of phenylalanine showed the greatest shootlet length (6.83 cm). Moreover, the explant grown on MS media with 200 mg/L of tryptophan had the largest number of leaves (16.32).Regarding fresh weight, the control group recorded the highest fresh plant weight 3.50 g, while the highest fresh weight of callus was 4.7 g under the treatment with tryptophan at 100 mg/L or glutamine at 300 mg/L. .The HPLC analysis of rutin for the callus treated with glutamine at 100 mg/L or phenylalanine 200 mg/L (the highest treatments of flavonoids) scored 88.2 and 64.3 µg/g, respectively. The toxicity of callus extract treated with glutamine at 100 mg/L was more toxic for cancer cells (HepG2) followed by phenylalanine at 200 mg/L at IC50 356.65 and 371.05 µg/mL, respectively. Additionally, in molecular docking, rutin effectively reduced the activity in the HepG2 promoter. It downregulated the mRNA expression of HepG2-responsive genes of liver cancer cells, reducing its capacity to form spheroids and blocking the HepG2 signaling pathway. These findings suggest that amino acid elicitors can improve the metabolism of Capparis spinosa L. and could be used to produce high amounts of rutin, which has antitumor activity against HepG2. Preventing oxidative damage and inducing apoptosis may be the mechanisms responsible for the anti-tumor effect. Rutin from Capparis spinosa presents a promising candidate for further in silico and in vitro anticancer research, particularly through molecular docking studies to explore its interactions with key oncogenic targets.
520		3	_aهدفت الدراسة إلى اختبار تأثير ثلاثة أحماض أمينية (تربتوفان، جلوتامين، فينيل ألانين) على نمو نبات الكباريس وإنتاج مركباته الثانوية ونشاطه المضاد للسرطان. أثبت محلول الكلوروكس 15% أعلى كفاءة تعقيم، بينما حقق BAP أعلى طول للساق والكينيتين أعلى عدد للأفرع. وأظهر التربتوفان 200 ملجم/لتر أفضل نمو من حيث عدد الأفرع، في حين أعطى الفينيل ألانين 100 ملجم/لتر أطول الأفرع. سجّل الجلوتامين 100 ملجم/لتر والفينيل ألانين 200 ملجم/لتر أعلى محتوى من الروتين. وأظهر الكالس المعالج بالجلوتامين 100 ملجم/لتر أقوى تأثير مضاد لخلايا سرطان الكبد HepG2. كما بيّن الربط الجزيئي قدرة الروتين على خفض التعبير الجيني للخلايا السرطانية وإيقاف مسار إشاراتها، مما يجعله مرشحًا واعدًا لأبحاث علاج السرطان.
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650		0	_aAgricultural Biochemistry
650		0	_aالكيمياء الحيوية الزراعية
653		1	_aCaper _aAnti cancer activity _aTissue culture _aFlavonoids _aSecondary metabolites _aMolecular docking _aالنشاط المضاد للسرطان _aالكباريس
700	0		_aSherif Helmy Ahmedm _ethesis advisor.
700	0		_aRania Saber Yousef _ethesis advisor.
700	0		_aShreif Said Saleh Ahmed _ethesis advisor.
900			_b01-01-2025 _cSherif Helmy Ahmedm _cRania Saber Yousef _cShreif Said Saleh Ahmed _dAmira Shawky Soliman _dHanaa Fawzy Mohamed _UCairo University _FFaculty of Agriculture _DDepartment of Agricultural Biochemistry
905			_aShimaa
942			_2ddc _cTH _e21 _n0
999			_c179683