000			05961namaa22004451i 4500
003			EG-GICUC
005			20260422183609.0
008			260422s2025 ua a|||frm||| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a576.5
092			_a576.5 _221
097			_aPh.D
099			_aCai01.07.10.Ph.D.2025.Ra.I
100	0		_aRasha Ahmed Ahmed Ali Elmoreigi, _epreparation.
245	1	0	_aIsolation of the dehydrin 1 (Dh1) gene from mangrove (Avicennia marina L) and transformation in apricot / _cby Rasha Ahmed Ahmed Ali Elmoreigi ; Supervisors Dr. Ebtissam Hussein Aly Hussein, Dr. Shereen Abu El-Maaty Mohamed, Dr. Sabah Anwar Hassanen.
246	1	5	_aعزل جين الديهيدرين 1 (Dh1) من المانجروف (Avicennia marina L.) ونقله إلى أصل من المشمش (Prunus armeniaca L.)
264		0	_c2025.
300			_a152 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (Ph.D)-Cairo University, 2025.
504			_aBibliography: pages 134-152.
520		3	_aImprovement of fruit trees to withstand abiotic stress is becoming a major goal to mitigate the threatening effects of the climatic changes on fruits productivity. Apricot (Prunus armeniaca L.) is one of the most economic deciduous stone fruit crops globally. This study aimed to develop an efficient in vitro regeneration protocol for the Egyptian Al-Amar apricot rootstock, and laying the foundation for its genetic enhancement through introducing a dehydrin gene isolated from mangrove (Avicennia marina L.) via the agrobacterium-mediated transformation system. Two types of explants, cotyledons and hypocotyls, were cultured on 48 woody plant medium (WPM) with various concentrations of TDZ, IBA or NAA to optimize shoot induction and regeneration. Cotyledons exhibited direct somatic organogenesis, with the highest bud induction (78%) achieved on WPM containing 13.62 µM TDZ + 2.46 µM IBA. While, hypocotyls underwent indirect regeneration through callus formation, with the callusing rate (96.87%) on WPM supplemented with 15.89 µM TDZ + 1.61 µM NAA. Shoot proliferation was most successful on media with 8.87 µM BAP and 0.54 µM NAA, with hypocotyls producing more shoots per explant than cotyledons. Optimal rooting occurred on WPM with 9.80 µM IBA. In parallel, the full-length of the dehydrin gene DHN was isolated from Avicennia marina via RT-PCR and cloned into pGEM-T Easy vector for sequencing and subsequently cloned into the pRI 201-AN plant expression vector. This construct was introduced into Agrobacterium tumefaciens LBA4404. Al-Amar cotyledon and hypocotyl explants were co- cultivated with Agrobacterium, followed by selection on media containing kanamycin and cefotaxime. For cotyledons, the highest survival rate (54.1%) was observed with 500 mg/l cefotaxime and 100 mg/l kanamycin and buds induction of 20.9% was achieved on 400 mg/l cefotaxime and 50 mg/l kanamycin. In contrast, hypocotyls showed better survival on 500 mg/l cefotaxime and 50 mg/l kanamycin, but had limited callus formation. PCR screening confirmed the presence of the DHN gene in transformed tissues. The transformation efficiency of buds derived from cotyledons and calli induced from hypocotyls was 1.04% and 0.50%, respectively. This study represents the first attempt to develop an Egyptian transgenic apricot rootstock with improved salinity tolereance which could pave the way for producing non-transgenic apricot fruits under adverse conditions via transgrafting.
520		3	_aتم تأسيس برتوكول فعال لإعادة التكشف معملياً لأصل المشمش المصري (العمار) باستخدام نوعين من المنفصلات النباتية وهي: الفلقات والتي أظهرت تخليق الأعضاء الجسدية المباشر دون تكوين كالس وقد بلغت أعلى نسبة لتكوين البراعم (78%) و المنفصلات السويقة الجنينية والتي اتجهت للتخليق الغير مباشر للأعضاء النباتية من خلال تكوين الكالس ثم البراعم حيث بلغت أعلى نسبة لتحفيز انتاج الكالس (96.87%)، بالتوازي تم عزل جين الديهيدرين (DHN) الكامل من نبات المانجروف باستخدام تقنية النسخ العكسي (RT-PCR) ، وكلونته في ناقل التعبير الجيني في النبات pRI 201-AN ثم ادخاله إلى بكتريا الأجروباكتريم والتي استخدمت لاحقاً في التحول الوراثي لأصل المشمش "العمار" و بلغت كفاءة التحول الوراثي للبراعم الناتجة من الفلقات والكالس الناتج من السويقات الجنينية 1.04% و 0.50% على التوالي.
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650		0	_aGenetics
650		0	_aالوراثة
653		1	_aApricot rootstock _aCotyledon _aHypocotyl _aExplant type, _aPrunus armeniaca _ain vitro regeneration _aDHN gene _aAvicennia marina _aإعادة تكشف النباتات معملياً _aأصل المشمش
700	0		_aEbtissam Hussein Aly Hussein _ethesis advisor.
700	0		_aShereen Abu El-Maaty Mohamed _ethesis advisor.
700	0		_aSabah Anwar Hassanen _ethesis advisor.
900			_b01-01-2025 _cEbtissam Hussein Aly Hussein _cShereen Abu El-Maaty Mohamed _cSabah Anwar Hassanen _dMohamed Abd Elsalam Rashed _dSalah Eldin Sayed Mohamed Elassal _UCairo University _FFaculty of Agriculture _DDepartment of Genetics
905			_aShimaa
942			_2ddc _cTH _e21 _n0
999			_c179684