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003			EG-GICUC
005			20260510132241.0
008			260510s2025 ua a|||frm||| 000 0 eng d
040			_aEG-GICUC _beng _cEG-GICUC _dEG-GICUC _erda
041	0		_aeng _beng _bara
049			_aDeposit
082	0	4	_a660.6
092			_a660.6 _221
097			_aM.Sc
099			_aCai01.07.06.M.Sc.2025.No.C
100	0		_aNora Rabee Gowda Abo El-kassem, _epreparation.
245	1	0	_aConservation of endangered medicinal plants natural resources through biotechnology techniques / _cby Nora Rabee Gowda Abo El-kassem ; Supervisors Dr. Sayed Abdel Kader Fayed, Dr. Mohamed AbdeL-Shakur, Dr. Mai Abdel - Moez Allam.
246	1	5	_aحفظ الموارد الطبيعية للنباتات الطبية المعرضة للانقراض من خلال تقنيات التكنولوجيا الحيوية
264		0	_c2025.
300			_a110 pages : _billustrations ; _c25 cm. + _eCD.
336			_atext _2rda content
337			_aUnmediated _2rdamedia
338			_avolume _2rdacarrier
502			_aThesis (Ph.D)-Cairo University, 2025.
504			_aBibliography: pages 89-110.
520		3	_a The study investigated the effects of fourth different shoot induction media on growth. The first medium was BAv medium, a combination of 0.5 mg L− 1 benzyl adenine (BA), 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic acid, and 0.5 mg L− 1 pyridoxine was added to the initial medium. The second medium was MD medium supplemented with 3 mg L− 1 BA, 0.2 mg L− 1 naphthaleneacetic acid (NAA), 10 mg L− 1 thiamine HCl, 1 mg L− 1 nicotinic acid, and 1 mg L− 1 pyridoxine. The third medium was ABv medium formulated with 1 mg L− 1 Adenine sulfate, 1 mg L− 1 benzyl adenine, 10 mg L− 1 thiamine HCl, 1 mg L− 1 nicotinic acid, and 1 mg L− 1 pyridoxine. The fourth medium AS1v contains 4.4 g L-¹ MS, 30 g L-¹ sucrose, 1 mg L-¹ adenine sulfate, 5 mg L-¹ thiamine HCl, 0.5 mg L-¹ nicotinic acid, 0.5 mg L-¹ pyridoxine, and 2.8 g L-¹ gelrite. and three media for rooting, the first medium was IB medium supplemented with 0.4 mg L− 1 indol butyric acid, 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic acid, and 0.5 mg L− 1 pyridoxine. The second medium was IA medium containing 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic acid, and 0.5 mg L− 1 pyridoxine. The third medium was NA media formulated with 0.4 mg L− 1 naphthaleneacetic acid, 5 mg L− 1 thiamine HCl, 0.5 mg L− 1 nicotinic acid, and 0.5 mg L− 1 pyridoxine. All media were prepared using Murashige and Skoog (MS) basal salts 4.4 g L− 1 supplemented with 30 g L− 1 sucrose, and solidified with 2.8 g L− 1 gelrite. Various propagation protocols were tested based on the availability of plant materials. HPLC analysis revealed that the ethanolic extract had the highest quantities of rutin (19.07 µg/mL) and vanillin (6.29 µg/mL), while the aqueous extract had the highest levels of gallic acid (7.02 µg/mL) and chlorogenic acid (6.02 µg/mL). SDS-PAGE examination revealed five protein bands ranging in molecular weight from 28 kDa to 240 kDa. DNA barcoding was used for molecular authentication, and the acquired sequence was matched to GenBank database sequences using the BLAST tool.
520		3	_aهدفت الدراسة إلى تقييم تأثير ثلاثة أوساط غذائية مختلفة على استحثاث الأفرع .وقد جرى اختبار عدة بروتوكولات إكثار وفقًا لتوافر المواد النباتية.أظهر تحليل HPLC أن المستخلص الإيثانولي احتوى على أعلى تركيز من الروتين (19.07 ميكروجرام/مل) والفانيلين (6.29 ميكروجرام/مل)، بينما سجل المستخلص المائي أعلى نسب من حمض الجاليك (7.02 ميكروجرام/مل) وحمض الكلوروجينيك (6.02 ميكروجرام/مل). كما أوضحت نتائج SDS-PAGE وجود خمسة نطاقات بروتينية تراوحت أوزانها الجزيئية بين 240 إلى 28 كيلودالتون. ولغرض التوثيق الجزيئي تم استخدام DNA barcoding، حيث تمت مقارنة التسلسل الناتج مع قاعدة بيانات GenBank باستخدام أداة BLAST
530			_aIssues also as CD.
546			_aText in English and abstract in Arabic & English.
650		0	_aBiotechnology Techniques
650		0	_aتقنيات التكنولوجيا الحيوية
653		1	_aMedicinal plants _aEndangered plants _aDNA barcoding _aSDS _aHPLC
700	0		_aSayed Abdel Kader Fayed _ethesis advisor.
700	0		_aMohamed AbdeL-Shakur _ethesis advisor.
700	0		_aMai Abdel - Moez Allam _ethesis advisor.
900			_b01-01-2025. _cSayed Abdel Kader Fayed _cMohamed AbdeL-Shakur _cMai Abdel - Moez Allam _UCairo University _FFaculty of Agriculture _DDepartment of Biochemistry
905			_aShimaa
942			_2ddc _cTH _e21 _n0
999			_c180075